Klapperich Lab:Notebook/Lab Meeting Notes/2010/03/18: Difference between revisions
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=18 March 2010 Lab Meeting= | |||
Jason Keller Presentation. | |||
'''‡ Announcements'''<br> | |||
'''‡ Flu R01:Integration''' <br> | |||
* meetings every other M 1-3pm. 705. <br> | |||
† HDA (Lead: Jaephil, Team:Sonali)<br> | |||
* Patient Samples - dilution experiments (MM/SH/JD) <br> | |||
* Start planning R01 for Submission on '''6/5/10.''' MM, JD, CMK. <br> | |||
* HDA mix in dried form - Expecting it from BH. <br> | |||
* warm start on the way - MTA (CMK)<br> | |||
* HDA negative water reactions all have primer dimers/non-specific band at 70bp for c diff HDA (MM)<br> | |||
* 2nd HDA design - "microSPE" + "Flu primer" + "HDA in a cup" + "Detection kit from Biohelix"<br> | |||
<br> | |||
† Sample Concentration (Lead: Jane, Team: Jaephil) <br> | |||
* Air resistance in the air channel is too large. <br> | |||
** New branched "membrane" chip design #1 in lithography stage. <br> | |||
** New "tube" chip design in drawing stage. Obtained more porous PTFE tubing samples <br> | |||
* Samples expected from Fauchet group. <br> | |||
<br> | |||
† SPE Column Optimization for DNA/RNA. (Lead: Sonali, Team: Hussam, Jessie, Brendan) <br> | |||
<br> | |||
* Jessie will run chips with serial virus dilutions from 1:1 to 1:1,000,000 to re-do previous dilution experimnent with WHO SYBR green assay on chip.<br> | |||
* Less input sample (same concentration). Results to come. (JC) <br> | |||
* Casein in human serum experiments ongoing. (HM) <br> | |||
<br> | |||
† Integrated chip for flu(SPE+RT+PCR) (Lead: Qingqing) <br> | |||
* Real copy number of previous human samples. So QQ is working with the higher concentration samples. Range is 10^7-10^9 of copy number/ml. <br> | |||
* REVISIT the % recovery number. <br> | |||
* QQ will try: more enzyme, Tween 20, BSA amounts. <br> | |||
<br> | |||
* PCR1 will combine with PCR2 if PCR2 comes back for revisions. (CMK)<br> | |||
<br> | |||
'''‡C. diff Project''' (Cathie, Sonali, Satish Singh, Lisa J., His post doc )<br> | |||
* See email notes. <br> | |||
<br> | |||
† PCR of C.Difficile DNA (Qingqing)<br> | |||
* | |||
<br> | |||
'''‡ Coulter Flu Fraunhofer Project (Lead: Sonali, Team: Sonali, Jessie, Cathie, CMI Folks, Qingqing)''' | |||
* New meeting time, T, 9:30 am every other week. MEETINGS CANCELLED THIS WEEK. <br> | |||
<br> | |||
'''‡ Agilent Automated Sample Preparation (Lead: Alex)'''<br> | |||
* Paper on HotDog - with CMK/ASB <br> | |||
<br> | |||
'''‡ COBRA (Lead: Jaephil, Team: Cathie, Jane for virus only, PHO folks)<br>''' | |||
* Evap paper - Additional experiment for Fig 3. By 3/15. <br> | |||
<br> | |||
'''‡ Biointerfaces group (Lead: MinCheol, Team: Cathie, MCK, Wong and Meller folks)'''<br> | |||
'''* Cathie will submit paper.<br>''' | |||
<br> | |||
'''‡ CIMIT- Sepsis (Lead:Cathie, Team:TBA)'''<br> | |||
* Sample collection ongoing. <br> | |||
<br> | |||
'''‡ PATH Grant (Lead:Cathie, Team:Frank, Sean, Mark, Jake Trueb (ME))''' <br> | |||
* Phone call with PATH 3/22. <br> | |||
* Frank: One week data up 3/5/10..<br> | |||
* Sean: Started making straws for the new experiments we talked about (DNA weights of 1ng, 10ng, and 100ng through 700nm at SPE 100 monolith at 0-day time point). Testing has been very slow-going due to an excess amount of other school work/tests. <br> | |||
* Mark: as of 3/4/10 up to 10% recovery from 10 ng input sample with water elution.<br> | |||
<br> | |||
'''‡ IIH Senior Project.'''<br> | |||
* Finished with 100 micrometer films. Registration experiments. "Optimized." <br> | |||
* 44 micron films. COP. This week. <br> | |||
* Elisa Test still on to do list. <br> | |||
Revision as of 14:54, 17 March 2010
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18 March 2010 Lab MeetingJason Keller Presentation. ‡ Announcements ‡ Flu R01:Integration
† HDA (Lead: Jaephil, Team:Sonali)
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