Klapperich Lab:Notebook/Lab Meeting Notes/2010/02/04: Difference between revisions
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* Hussam Runs chip with RNA (confirm procedure is correct) [update]<br> | * Hussam Runs chip with RNA (confirm procedure is correct) [update]<br> | ||
* Jessie will run chips with serial virus dilutions from 1:1 to 1:1,000,000 to re-do previous dilution experimnent with WHO SYBR green assay on chip.<br> | * Jessie will run chips with serial virus dilutions from 1:1 to 1:1,000,000 to re-do previous dilution experimnent with WHO SYBR green assay on chip.<br> | ||
* Jessie will test 2mm wide channels with 4uL SPE with Black Beauty for efficiency. <br> | |||
* Sonali will assay BIDMC virus sample with newest protocol with 0.7uM Silica and compare with Qiagen RNA extraction with CDC PCR assay <br> | * Sonali will assay BIDMC virus sample with newest protocol with 0.7uM Silica and compare with Qiagen RNA extraction with CDC PCR assay <br> | ||
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'''‡ Coulter Flu Fraunhofer Project (Lead: Sonali, Team: Sonali, Jessie, Cathie, CMI Folks, Qingqing)''' | '''‡ Coulter Flu Fraunhofer Project (Lead: Sonali, Team: Sonali, Jessie, Cathie, CMI Folks, Qingqing)''' | ||
* New meeting time, T, 9:30 am every other week. <br> | * New meeting time, T, 9:30 am every other week. <br> | ||
* Will be done by next Tuesday - PCR with 3 different kits of same serial dilution RNA sample (Invitrogen - SuperscriptIII Platinum, Ambion - AgPath-ID, Qiagen - OneStep RT-PCR Kit)<br> | |||
<br> | <br> | ||
'''‡ Agilent Automated Sample Preparation (Lead: Alex)'''<br> | '''‡ Agilent Automated Sample Preparation (Lead: Alex)'''<br> | ||
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'''‡ IIH Senior Project.'''<br> | '''‡ IIH Senior Project.'''<br> | ||
* | * ELISA kits have been delivered | ||
* | * Protocol written for using cutter plotter. Will post to share drive | ||
** | * Made "test" chips from 100 micron COP but cuts are sloppy | ||
** | ** Will change settings on cutter to clean up cuts | ||
* | *** First thought is to slow down speed | ||
* | * Started acquiring images of the 100 micron "test" chips | ||
Revision as of 12:28, 4 February 2010
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4 February 2010 Lab MeetingQingqing Prospectus Practice Presentation. ‡ Announcements
† HDA (Lead: Jaephil, Team:Sonali)
† Sample Concentration (Lead: Jane, Team: Jaephil)
† SPE Column Optimization for DNA/RNA. (Lead: Sonali, Team: Hussam, Jessie, Brendan)
† integrated chip for flu(SPE+RT+PCR) (Lead: Qingqing)
† PCR of C.Difficile DNA (Qingqing)
-wrong size products(55bp). -no designed size product.
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