Kim:Research: Difference between revisions
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<font face="trebuchet ms" size=3 style="color:#000">'''Technological:</font> <font face="trebuchet ms" size=3 style="color:#00688B">Micro- and Nanoscale engineering of biomimetic in vitro cell culture models and functional tissue scaffolds </font> <br> </div> | <font face="trebuchet ms" size=3 style="color:#000">'''Technological:</font> <font face="trebuchet ms" size=3 style="color:#00688B">Micro- and Nanoscale engineering of biomimetic in vitro cell culture models and functional tissue scaffolds </font> <br> </div> | ||
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Our current research focuses on engineering combinatorial cellular microenvironment through use of variable nano-patterns, and soluble and matrix-bound cell guidance cues in a single experiment, which better mimics the in vivo microenvironment under physiological conditions. For example, we are developing a microfluidics-based on chip assay integrated with complex nanoscale topographic features to enable the analysis of concerted cell responses to composite gradients of precisely generated and aligned surface-bound ECM molecules and diffusible guidance cues or topographic guidance cues. Using these tools, we strive to systematically characterize live cells to wide spectra of dynamically changing combination of mechanical and chemical stimuli (e.g. ECM proteins, topographic, growth factors and signal transduction pathway inhibitors). The proposed measurements are highly resolved in time and space, using a variety of live cell probes and highly defined extracellular conditions. In collaboration with other nanofabrication groups, we are developing nanotopography-integrated cell culture systems and biomaterial tissue scaffolds using UV-assisted capillary force lithography and/or nanoimprinting techniques. For high-throughput analysis, we are also working to combine a large area nanopatterned substrate with a traditional multi-well tissue culture plate. We aim to use these tools to gain new mechanistic insights into cell signaling and function, to design new therapies or diagnostic tests for cancer progression and cardiovascular diseases, and to establish organizing principles for development of precisely defined scaffolds for advanced tissue engineering applications. | Our current research focuses on engineering combinatorial cellular microenvironment through use of variable nano-patterns, and soluble and matrix-bound cell guidance cues in a single experiment, which better mimics the in vivo microenvironment under physiological conditions. For example, we are developing a microfluidics-based on chip assay integrated with complex nanoscale topographic features to enable the analysis of concerted cell responses to composite gradients of precisely generated and aligned surface-bound ECM molecules and diffusible guidance cues or topographic guidance cues. Using these tools, we strive to systematically characterize live cells to wide spectra of dynamically changing combination of mechanical and chemical stimuli (e.g. ECM proteins, topographic, growth factors and signal transduction pathway inhibitors). The proposed measurements are highly resolved in time and space, using a variety of live cell probes and highly defined extracellular conditions. In collaboration with other nanofabrication groups, we are developing nanotopography-integrated cell culture systems and biomaterial tissue scaffolds using UV-assisted capillary force lithography and/or nanoimprinting techniques. For high-throughput analysis, we are also working to combine a large area nanopatterned substrate with a traditional multi-well tissue culture plate. We aim to use these tools to gain new mechanistic insights into cell signaling and function, to design new therapies or diagnostic tests for cancer progression and cardiovascular diseases, and to establish organizing principles for development of precisely defined scaffolds for advanced tissue engineering applications. | ||
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Revision as of 10:36, 2 November 2010
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