http://openwetware.org/index.php?title=Killing_Buffer&feed=atom&action=history 2013-06-19T08:09:21Z Revision history for this page on the wiki MediaWiki 1.13.2 http://openwetware.org/index.php?title=Killing_Buffer&diff=263687&oldid=prev 2008-11-20T10:45:37Z

<p>New page: ==Purpose== *Killing Buffer is used to kill bacterial cells. Usually one would use cold Killing buffer in a 50:50 ratio to harvest cells in liquid medium. The buffer contains NaN3 and kill...</p> <p><b>New page</b></p><div>==Purpose==<br /> *Killing Buffer is used to kill bacterial cells. Usually one would use cold Killing buffer in a 50:50 ratio to harvest cells in liquid medium. The buffer contains NaN3 and kills the cells quickly. Fast killing and down cooling is important for example to stop RNases if you want to isolate RNA from that specific time point of cell growth. <br /> <br /> ==Recipe==<br /> <br /> *Endconzentration will be 20mM Tris and 5mM MgCl&lt;sub&gt;2&lt;/sub&gt; and 20mM NaN&lt;sub&gt;3&lt;/sub&gt; <br /> <br /> {|border=&quot;1&quot;<br /> |+ '''Killing Buffer'''<br /> ! for 1 liter <br /> |-<br /> |2 ml 1M Tris-HCl pH 7.5 <br /> |-<br /> |0.5 ml 1M MgCl&lt;sub&gt;2&lt;/sub&gt; <br /> |-<br /> |1.3 g NaN&lt;sub&gt;3&lt;/sub&gt;<br /> |-<br /> |997.5 ml ddH&lt;sub&gt;2&lt;/sub&gt;O <br /> |}<br /> <br /> *Store at 4°C.<br /> <br /> *Note that NaN&lt;sub&gt;3&lt;/sub&gt; is highly toxic and should be handled accordingly.<br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> [[Category:Material]] [[Category:Buffers]]</div>

Torsten Waldminghaus