Ketner Lab

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Antibodies)
Line 25: Line 25:
==Materials==
==Materials==
===Antibodies===
===Antibodies===
-
*anti-HA
+
*[[anti-HA]]
-
*anti-Fiber
+
*[[anti-Fiber]]
-
*anti-Late serum
+
*[[anti-Late serum]]
-
*anti-NANP hybridoma supernatant
+
*[[anti-NANP hybridoma supernatant]]
== Links ==
== Links ==

Revision as of 11:19, 6 September 2006

Image:JHSPHbanner.jpg


Contents

Current Projects

Adenovirus Vaccines

Ubiquination


Protocols

  • Adenovirus CaCl transfection
  • Generating hexon mutants in E. coli
  • Generating fiber mutants in E. coli
  • Generating late gene expression mutants in 293 cells
  • Purifying adenovius (banded stocks)
  • Titration of viral stocks (calculate pfu)
  • Calculate MOI
  • Protein preps of infected 293 cells for westerns
  • Western Blots
  • P32 label DNA

Materials

Antibodies

Links

Gary Ketner

JHSPH Magazine

Personal tools