Ketner Lab

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(Protocols)
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*Calculate MOI
*Calculate MOI
*Protein preps of infected 293 cells for westerns
*Protein preps of infected 293 cells for westerns
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*Western Blots
+
*[[Western Blots]]
*P32 label DNA
*P32 label DNA
-
 
== Links ==
== Links ==

Revision as of 11:00, 31 August 2006

Image:JHSPHbanner.jpg


Current Projects

Adenovirus Vaccines

Ubiquination


Protocols

  • Adenovirus CaCl transfection
  • Generating hexon mutants in E. coli
  • Generating fiber mutants in E. coli
  • Generating late gene expression mutants in 293 cells
  • Purifying adenovius (banded stocks)
  • Titration of viral stocks (calculate pfu)
  • Calculate MOI
  • Protein preps of infected 293 cells for westerns
  • Western Blots
  • P32 label DNA

Links

Gary Ketner

JHSPH Magazine

Personal tools