Ketner Lab: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(Creation of the Ketner lab page) |
mNo edit summary |
||
(9 intermediate revisions by one other user not shown) | |||
Line 1: | Line 1: | ||
[[Image:JHSPHbanner.jpg]] | [[Image:JHSPHbanner.jpg]] | ||
---- | ---- | ||
Line 6: | Line 5: | ||
== Current Projects == | == Current Projects == | ||
'''Adenovirus Vaccines''' | '''Adenovirus Vaccines''' | ||
*Malaria | *[[Malaria]] | ||
*HPV | *HPV | ||
'''Ubiquination''' | '''Ubiquination''' | ||
== Protocols == | == Protocols == | ||
Line 23: | Line 20: | ||
*Calculate MOI | *Calculate MOI | ||
*Protein preps of infected 293 cells for westerns | *Protein preps of infected 293 cells for westerns | ||
*Western | *[http://www.openwetware.org/index.php?title=Ketner:_Tissue_culture_viral_lysate_Western_Blot Western Blot] | ||
*P32 label DNA | *P32 label DNA | ||
*Quantitate number of virions in CsCl virus preps | |||
==Materials== | |||
===Antibodies=== | |||
*[[anti-HA]] | |||
*[[anti-Fiber]] | |||
*[[anti-Late serum]] | |||
*[[anti-NANP hybridoma supernatant]] | |||
===Plasmids=== | |||
===Viruses=== | |||
===Cells=== | |||
== Links == | |||
[http://faculty.jhsph.edu/?F=Gary&L=Ketner Gary Ketner] | |||
[http://www.jhsph.edu/PublicHealthNews/Magazine/ JHSPH Magazine] |
Latest revision as of 12:55, 20 April 2008
Current Projects
Adenovirus Vaccines
- Malaria
- HPV
Ubiquination
Protocols
- Adenovirus CaCl transfection
- Generating hexon mutants in E. coli
- Generating fiber mutants in E. coli
- Generating late gene expression mutants in 293 cells
- Purifying adenovius (banded stocks)
- Titration of viral stocks (calculate pfu)
- Calculate MOI
- Protein preps of infected 293 cells for westerns
- Western Blot
- P32 label DNA
- Quantitate number of virions in CsCl virus preps