Kathleen McGinness

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Contents

Work Experience

Education

  • B.S. in Psychology, University of Maryland College Park, 1996

Science stuff I know something about...

  • tmRNA (or SsrA/10Sa RNA for you purists)
  • translation
  • energy-dependent proteolysis in bacteria
  • The RNA World
  • molecular biology in a world without kits
  • in vitro selection and evolution of nucleic acids
  • generation of nucleic acid and protein libraries
  • in vitro RNA techniques
  • nucleic acid-protein interactions (in vitro analysis and bacterial in vivo analysis)
  • fishing experiments to look for things that interact with your RNA/DNA/protein of interest
  • PCR (even colony PCR, although it is not a separate skill)
  • bacterial protein expression/purification
  • in vivo assays with E. coli i.e. analyzing effects on proteins/RNA
  • molecular cloning
  • Western/Northern/Southern blot analysis
  • oligonucleotide synthesis
  • RNA/DNA sequencing ("old school" and "new school" techniques)
  • chemical and enzymatic structural probing of RNA/DNA
  • gels, all kinds of gels: agarose, native acrylamide, denaturing acrylamide, SDS-acrylamide, two-dimensional
  • enzyme kinetics
  • radiolabeling nucleic acids and proteins
  • familiarity with some biophysical techniques (a bit of CD spectroscopy, a small amount of analytical ultracentrifugation)
  • fluorescence spectroscopy (nothing too fancy: straight-up fluorescence and a bit of anisotropy)

Publications

Engineering controllable protein degradation.

McGinness, K.E., Baker, T.A. & Sauer, R.T. (2006) Mol. Cell, 22, 701-707.

Ribosomal protein S1 binds mRNA and tmRNA similarly but plays distinct roles in translation of these molecules.

McGinness, K.E. & Sauer, R.T. (2004) Proc. Natl. Acad. Sci. USA 101, 13454–13459.

A Glimpse into tmRNA-mediated Ribosome Rescue.

Moore, S.D., McGinness, K.E. & Sauer, R.T. (2003) Science 300, 127–130. (comment)

In Search of an RNA Replicase Ribozyme.

McGinness, K.E. & Joyce, G.F. (2003) Chem. Biol. 10, 5–14. (review)

Continuous In Vitro Evolution of a Ribozyme that Catalyzes Three Successive Nucleotidyl Addition Reactions.

McGinness, K.E., Wright, M.C. & Joyce, G.F. (2002) Chem. Biol. 9, 585–596.

RNA-Catalyzed RNA Ligation on an External RNA Template.

McGinness, K.E. & Joyce, G.F. (2002) Chem. Biol. 9, 297–307.

Substitution of Ribonucleotides in the T7 RNA Polymerase Promoter Element.

McGinness, K.E. & Joyce, G.F. (2002) J. Biol. Chem. 277, 2987–2991.

Contact

If you're on OWW, post a message on my talk page, or send me an email.

If you're not on OWW, join now!

Fun facts

  • Who's visiting? (Thanks Barry!)

Locations of visitors to this page

Important things I've learned through real life experiences.

  • If you can't fix it with duct tape, a safety pin, or both, you should probably just throw it out.
  • Almost everything is better with bacon.
  • Never underestimate the importance of your karass (although I like this definition better) and beware of granfalloons.
  • Very few people know who Foghorn Leghorn is.

"Personal"

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