Kathleen McGinness

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
m
Line 111: Line 111:
</html>
</html>
-
*Other former WFJL crew on OWW: [[Roshan Kumar]], [[User:Wmshih|William Shih]]
+
*Here is an excellent tutorial on how [http://video.google.com/videoplay?docid=-2657697036715872139 translation] works, with an introduction by the 1971 version of nobel laureate [http://nobelprize.org/chemistry/laureates/1980/berg-cv.html Paul Berg]. I'd like to see it in classrooms everywhere. '''tRNA! peptide bond!'''
-
**Related former TSRI folk on OWW: [[Plachikkat Radha]]
+
-
 
+
-
===Important things I've learned through real life experiences.===
+
-
*If you can't fix it with duct tape, a safety pin, or both, you should probably just throw it out.
+
-
*Almost everything is better with bacon.
+
-
*Lots of things are better with a poached or fried egg on top.
+
-
*Never underestimate the importance of your [http://en.wikipedia.org/wiki/Karass karass] (although I like [http://oook.info/bloggery/karass.html this] definition better) and beware of [http://en.wikipedia.org/wiki/Granfalloon granfalloons].
+
-
*Very few people know who [http://en.wikipedia.org/wiki/Foghorn_Leghorn Foghorn Leghorn] is.
+
[[KM:Personal|<font size=4>"Personal"</font>]]
[[KM:Personal|<font size=4>"Personal"</font>]]

Revision as of 23:54, 14 February 2009

Contents

Work Experience

Education

  • B.S. in Psychology, University of Maryland College Park, 1996

Science stuff I know something about...

  • tmRNA (or SsrA/10Sa RNA for you purists)
  • translation
  • energy-dependent proteolysis in bacteria
  • The RNA World
  • molecular biology in a world without kits
  • in vitro selection and evolution of nucleic acids
  • generation of nucleic acid and protein libraries
  • in vitro RNA techniques
  • nucleic acid-protein interactions (in vitro analysis and bacterial in vivo analysis)
  • fishing experiments to look for things that interact with your RNA/DNA/protein of interest
  • PCR (even colony PCR, although it is not a separate skill)
  • bacterial protein expression/purification
  • in vivo assays with E. coli i.e. analyzing effects on proteins/RNA
  • molecular cloning
  • Western/Northern/Southern blot analysis
  • oligonucleotide synthesis
  • RNA/DNA sequencing ("old school" and "new school" techniques)
  • chemical and enzymatic structural probing of RNA/DNA
  • gels, all kinds of gels: agarose, native acrylamide, denaturing acrylamide, SDS-acrylamide, two-dimensional
  • enzyme kinetics
  • radiolabeling nucleic acids and proteins
  • familiarity with some biophysical techniques (a bit of CD spectroscopy, a small amount of analytical ultracentrifugation)
  • fluorescence spectroscopy (nothing too fancy: straight-up fluorescence and a bit of anisotropy)

Publications

Altered tethering of the SspB adaptor to the ClpXP protease causes changes in substrate delivery.

McGinness K.E., Bolon, D.N., Kaganovich, M., Baker, T.A. & Sauer, R.T. (2007) J. Biol. Chem., 282, 11465-11473. Epub 2007 Feb 22.

Engineering controllable protein degradation.

McGinness, K.E., Baker, T.A. & Sauer, R.T. (2006) Mol. Cell, 22, 701-707.

Ribosomal protein S1 binds mRNA and tmRNA similarly but plays distinct roles in translation of these molecules.

McGinness, K.E. & Sauer, R.T. (2004) Proc. Natl. Acad. Sci. USA 101, 13454–13459.

A Glimpse into tmRNA-mediated Ribosome Rescue.

Moore, S.D., McGinness, K.E. & Sauer, R.T. (2003) Science 300, 127–130. (comment)

In Search of an RNA Replicase Ribozyme.

McGinness, K.E. & Joyce, G.F. (2003) Chem. Biol. 10, 5–14. (review)

Continuous In Vitro Evolution of a Ribozyme that Catalyzes Three Successive Nucleotidyl Addition Reactions.

McGinness, K.E., Wright, M.C. & Joyce, G.F. (2002) Chem. Biol. 9, 585–596.

RNA-Catalyzed RNA Ligation on an External RNA Template.

McGinness, K.E. & Joyce, G.F. (2002) Chem. Biol. 9, 297–307.

Substitution of Ribonucleotides in the T7 RNA Polymerase Promoter Element.

McGinness, K.E. & Joyce, G.F. (2002) J. Biol. Chem. 277, 2987–2991.

Contact

If you're on OWW, post a message on my talk page, or send me an email.

If you're not on OWW, join now!

Fun facts

  • Who's visiting? (Thanks Barry!)

Locations of visitors to this page

  • Here is an excellent tutorial on how translation works, with an introduction by the 1971 version of nobel laureate Paul Berg. I'd like to see it in classrooms everywhere. tRNA! peptide bond!

"Personal"

Personal tools