Kathleen McGinness

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==Work Experience==
 
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*Scientist, [http://www.archemix.com/ Archemix Corp.] (2006–??)
 
*Postdoc in the [http://mit.edu/sauerlab/ Sauer lab] at [http://web.mit.edu/ MIT] (2001–2006)
*Postdoc in the [http://mit.edu/sauerlab/ Sauer lab] at [http://web.mit.edu/ MIT] (2001–2006)
**[[Sauer Lab|Sauer lab OWW page]]
**[[Sauer Lab|Sauer lab OWW page]]
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==Education==
 
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*Ph.D. in Chemistry, [http://www.scripps.edu/e_index.html The Scripps Research Institute], 2001 (with [http://www.scripps.edu/mb/joyce/joycelab.html Gerald Joyce])
 
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*B.S. in Chemistry, [http://www.umd.edu University of Maryland College Park], 1996 (with [http://biophysics.jhu.edu/woodson/index.html Sarah Woodson])
 
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*B.S. in Psychology, University of Maryland College Park, 1996
 
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== Science stuff I know something about... ==
 
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*tmRNA (or SsrA/10Sa RNA for you purists)
 
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*translation
 
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*energy-dependent proteolysis in bacteria
 
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*The RNA World
 
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*molecular biology in a world without kits
 
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*''in vitro'' selection and evolution of nucleic acids
 
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*generation of nucleic acid and protein libraries
 
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*''in vitro'' RNA techniques
 
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*nucleic acid-protein interactions (''in vitro'' analysis and bacterial ''in vivo'' analysis)
 
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*fishing experiments to look for things that interact with your RNA/DNA/protein of interest
 
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*PCR (even colony PCR, although it is '''not''' a separate skill)
 
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*bacterial protein expression/purification
 
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*''in vivo'' assays with ''E. coli'' i.e. analyzing effects on proteins/RNA
 
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*molecular cloning
 
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*Western/Northern/Southern blot analysis
 
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*oligonucleotide synthesis
 
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*RNA/DNA sequencing ("old school" and "new school" techniques)
 
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*chemical and enzymatic structural probing of RNA/DNA
 
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*gels, all kinds of gels: agarose, native acrylamide,  denaturing acrylamide, SDS-acrylamide, two-dimensional
 
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*enzyme kinetics
 
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*radiolabeling nucleic acids and proteins
 
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*familiarity with some biophysical techniques (a bit of CD spectroscopy, a small amount of analytical ultracentrifugation)
 
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*fluorescence spectroscopy (nothing too fancy: straight-up fluorescence and a bit of anisotropy)
 
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==Publications==
 
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'''[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&dopt=AbstractPlus&list_uids=17317664&query_hl=1&itool=pubmed_docsum Altered tethering of the SspB adaptor to the ClpXP protease causes changes in substrate delivery.]'''
 
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McGinness K.E., Bolon, D.N., Kaganovich, M., Baker, T.A. & Sauer, R.T. (2007) J. Biol. Chem., 282, 11465-11473. Epub 2007 Feb 22.
 
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'''[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=16762842&query_hl=1&itool=pubmed_docsum Engineering controllable protein degradation.]'''
 
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McGinness, K.E., Baker, T.A. & Sauer, R.T. (2006) Mol. Cell, 22, 701-707.
 
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'''[http://www.pnas.org/cgi/content/full/101/37/13454 Ribosomal protein S1 binds mRNA and tmRNA similarly but plays distinct roles in translation of these molecules.]'''
 
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McGinness, K.E. & Sauer, R.T. (2004) Proc. Natl. Acad. Sci. USA 101, 13454–13459.
 
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'''[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12677051&query_hl=1 A Glimpse into tmRNA-mediated Ribosome Rescue.]'''
 
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Moore, S.D., McGinness, K.E. & Sauer, R.T. (2003)  Science 300, 127–130. (comment)
 
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'''[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12573693&query_hl=1 In Search of an RNA Replicase Ribozyme.]'''
 
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McGinness, K.E. & Joyce, G.F. (2003)  Chem. Biol. 10, 5–14. (review)
 
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'''[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12031665&query_hl=1 Continuous In Vitro Evolution of a Ribozyme that Catalyzes Three Successive Nucleotidyl Addition Reactions.]'''
 
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McGinness, K.E., Wright, M.C. & Joyce, G.F. (2002)  Chem. Biol. 9, 585–596.
 
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'''[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11927255&query_hl=1 RNA-Catalyzed RNA Ligation on an External RNA Template.]'''
 
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McGinness, K.E. & Joyce, G.F. (2002)  Chem. Biol. 9, 297–307.
 
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'''[http://www.jbc.org/cgi/content/full/277/4/2987 Substitution of Ribonucleotides in the T7 RNA Polymerase Promoter Element.]'''
 
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McGinness, K.E. & Joyce, G.F. (2002)  J. Biol. Chem. 277, 2987–2991.
 
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==Contact==
 
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If you're on [[Main Page|OWW]],  post a message on my [[User talk:Kathmc|talk page]], or send me an [[Special:Emailuser/Kathmc|email]].<br>
 
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If you're not on OWW, [[OpenWetWare: How to join|join now]]! <br>
 
==Fun facts==
==Fun facts==
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*[[Special:Contributions/Kathmc|My OWW contributions.]]
 
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*'''Who's visiting?''' (Thanks [[Barry Canton|Barry]]!)
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*'''Who's visiting?'''
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*Here is an excellent tutorial on how [http://video.google.com/videoplay?docid=-2657697036715872139 translation] works, with an introduction by the 1971 version of nobel laureate [http://nobelprize.org/chemistry/laureates/1980/berg-cv.html Paul Berg]. I'd like to see it in classrooms everywhere. '''tRNA! peptide bond!'''
*Here is an excellent tutorial on how [http://video.google.com/videoplay?docid=-2657697036715872139 translation] works, with an introduction by the 1971 version of nobel laureate [http://nobelprize.org/chemistry/laureates/1980/berg-cv.html Paul Berg]. I'd like to see it in classrooms everywhere. '''tRNA! peptide bond!'''
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[[KM:Personal|<font size=4>"Personal"</font>]]
 

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  • Here is an excellent tutorial on how translation works, with an introduction by the 1971 version of nobel laureate Paul Berg. I'd like to see it in classrooms everywhere. tRNA! peptide bond!
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