Karina Alvarez Week 2

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This week's assignment is to evaluate this primary research article.

Biological Terms

In order to fully understand the paper, I researched the terms that were not very familiar to me. These terms are defined here:

  • glutamate: major fast excitatory neurotransmitter in the mammalian central nervous system (Source)
  • glutamine: a crystalline amino acid occurring in proteins; important in protein metabolism (Source)
  • dehydrogenase: enzyme that oxidizes a substrate by transferring hydrogen to an acceptor that is either NAD/NADP or a flavin enzyme; removes hydrogen from its substrate (Source)
  • proline: amino acid abundant in collagens more than other proteins (Source)
  • Northern analysis: a procedure similar to the southern blot analysis, used mostly to separate and identify rNA fragments (Source)
  • permease: general term for a membrane protein that increases the permeability of the plasma membrane to a particular molecule by a process not requiring metabolic energy (Source)
  • oligonucleotides: polymers made up of a few nucleotides; in genetics, a short sequence synthesized to match a region where a mutation is known to occur, and then used as a probe (Source)
  • transferase: an enzyme that transfers a specific grouping from one molecule to another (Source)
  • flux: the total amount of a quantity passing through a given surface per unit time (Source)
  • metabolites: any substance produced by metabolism or by a metabolic process (Source)

Outline

Main Result

The main result of this study is that the concentration of ammonia, rather than the rate of input, is the limiting factor in the growth of Saccharomyces cerevisiae. This was based off much previous research indicating that the concentration of ammonia, more than any other nitrogen sources (including proline and urea, leads to more rapid growth in Saccharomyces cerevisiae.

Methods

Figures

Figure 1

Figure 2

Figure 3

Limitations

Significance

Main Result

Significance

Limitations

Methods

Helpful Links

BIOL398-04/S15:Week 2
BIOL398-04/S15:Class Journal Week 2

Karina Alvarez Main Page: Karina Alvarez
Math 388-01 Course Page: BIOL398-04/S15