Kanamycin: Difference between revisions
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==Mechanism of Resistance== | ==Mechanism of Resistance== | ||
Kanamycin is inactivated by bacterial aminophosphotransferases (APHs). The APHs inactivate kanamycin by transferring the γ-phosphate of ATP to the hydroxyl group in the 3' position of the pseudosaccharide. The ''Kan<sup>R</sup>'' gene codes for kanamycin resistance. Several versions of this gene exist, with varying crossover resistance to other antibiotics such as [[neomycin]]. | Kanamycin is inactivated by bacterial aminophosphotransferases (APHs). The APHs inactivate kanamycin by transferring the γ-phosphate of ATP to the hydroxyl group in the 3' position of the pseudosaccharide. The ''Kan<sup>R</sup>'' gene codes for kanamycin resistance. Several versions of this gene exist, with varying crossover resistance to other antibiotics such as [[neomycin]] or [[gentamycin]]. | ||
==Working Concentration and Stock Solution== | ==Working Concentration and Stock Solution== | ||
Revision as of 12:39, 19 December 2005
Mode of Action
Bacteriocidal. Diffuses through the porinchannels in the outer membrane of gram-negative bacteria. Interacts with at least three ribosomal proteins, inhibiting protein synthesis and increasing translation errors.
Mechanism of Resistance
Kanamycin is inactivated by bacterial aminophosphotransferases (APHs). The APHs inactivate kanamycin by transferring the γ-phosphate of ATP to the hydroxyl group in the 3' position of the pseudosaccharide. The KanR gene codes for kanamycin resistance. Several versions of this gene exist, with varying crossover resistance to other antibiotics such as neomycin or gentamycin.
Working Concentration and Stock Solution
Working concentration is 35 μg/ml. Stock solution is 35 mg/ml in water (kanamycin is insoluble in 50% alcohol).
Plate color code
Red stripe
References
Molecular Cloning: A Laboratory Manual, Vol 1.
