Kanamycin: Difference between revisions
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==Mode of Action== | ==Mode of Action== | ||
Diffuses through the porinchannels in the outer membrane of gram-negative bacteria. Interacts with at least three ribosomal proteins, inhibiting protein synthesis and increasing translation errors. | Diffuses through the porinchannels in the outer membrane of gram-negative bacteria. Interacts with at least three ribosomal proteins, inhibiting protein synthesis and increasing translation errors. | ||
==Mechanism of Resistance== | ==Mechanism of Resistance== | ||
Revision as of 09:25, 24 June 2005
Mode of Action
Diffuses through the porinchannels in the outer membrane of gram-negative bacteria. Interacts with at least three ribosomal proteins, inhibiting protein synthesis and increasing translation errors.
Mechanism of Resistance
Kanamycin is inactivated by bacterial aminophosphotransferases (APHs). The APHs inactivate kanamycin by transferring the γ-phosphate of ATP to the hydroxyl group in the 3' position of the pseudosaccharide.
References
Molecular Cloning: A Laboratory Manual, Vol 1.
