Kanamycin

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==Mode of Action==
==Mode of Action==
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Diffuses through the porinchannels in the outer membrane of gram-negative bacteria.  Interacts with at least three ribosomal proteins, inhibiting protein synthesis and increasing translation errors.
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Bacteriocidal.  Diffuses through the porinchannels in the outer membrane of gram-negative bacteria.  Interacts with at least three ribosomal proteins, inhibiting protein synthesis and increasing translation errors.
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==Mechanism of Resistance==
==Mechanism of Resistance==
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Kanamycin is inactivated by bacterial aminophosphotransferases (APHs). The APHs inactivate kanamycin by transferring the γ-phosphate of ATP to the hydroxyl group in the 3' position of the pseudosaccharide.
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Kanamycin is inactivated by bacterial aminophosphotransferases (APHs). The APHs inactivate kanamycin by transferring the &gamma;-phosphate of ATP to the hydroxyl group in the 3' position of the pseudosaccharide.  The ''Kan<sup>R</sup>'' gene codes for kanamycin resistance.  Several versions of this gene exist, with varying crossover resistance to other antibiotics such as [[neomycin]] or [[gentamycin]].
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==Working Concentration and Stock Solution==
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Working concentration is 50 &mu;g/ml, or 25 &mu;g/ml for low-copy plasmids like [http://openwetware.org/wiki/Bacterial_artificial_chromosomes#PAC_vectors PACs].  Stock solution is 35 mg/ml in water (kanamycin is insoluble in 50% alcohol).
==References==
==References==
Molecular Cloning: A Laboratory Manual, Vol 1.
Molecular Cloning: A Laboratory Manual, Vol 1.
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==Links==
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[http://bacpac.chori.org/clone-handling.htm Children's Hospital Oakland Research Institute (CHORI) - BAC/PAC resources]
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[[Category:Material]] [[Category:Antibiotic]]

Current revision

Contents

Mode of Action

Bacteriocidal. Diffuses through the porinchannels in the outer membrane of gram-negative bacteria. Interacts with at least three ribosomal proteins, inhibiting protein synthesis and increasing translation errors.

Mechanism of Resistance

Kanamycin is inactivated by bacterial aminophosphotransferases (APHs). The APHs inactivate kanamycin by transferring the γ-phosphate of ATP to the hydroxyl group in the 3' position of the pseudosaccharide. The KanR gene codes for kanamycin resistance. Several versions of this gene exist, with varying crossover resistance to other antibiotics such as neomycin or gentamycin.

Working Concentration and Stock Solution

Working concentration is 50 μg/ml, or 25 μg/ml for low-copy plasmids like PACs. Stock solution is 35 mg/ml in water (kanamycin is insoluble in 50% alcohol).

References

Molecular Cloning: A Laboratory Manual, Vol 1.

Links

Children's Hospital Oakland Research Institute (CHORI) - BAC/PAC resources

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