Kanamycin
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==Mode of Action== | ==Mode of Action== | ||
| - | + | Diffuses through the porinchannels in the outer membrane of gram-negative bacteria. Interacts with at least three ribosomal proteins, inhibiting protein synthesis and increasing translation errors. | |
==Mechanism of Resistance== | ==Mechanism of Resistance== | ||
| - | Kanamycin is inactivated by bacterial aminophosphotransferases (APHs). The APHs inactivate kanamycin by transferring | + | Kanamycin is inactivated by bacterial aminophosphotransferases (APHs). The APHs inactivate kanamycin by transferring γ -phosphate of ATP to the hydroxyl group in the 3' position of the pseudosaccharide. |
==References== | ==References== | ||
Molecular Cloning: A Laboratory Manual, Vol 1. | Molecular Cloning: A Laboratory Manual, Vol 1. | ||
Revision as of 12:24, 24 June 2005
Mode of Action
Diffuses through the porinchannels in the outer membrane of gram-negative bacteria. Interacts with at least three ribosomal proteins, inhibiting protein synthesis and increasing translation errors.
Mechanism of Resistance
Kanamycin is inactivated by bacterial aminophosphotransferases (APHs). The APHs inactivate kanamycin by transferring γ -phosphate of ATP to the hydroxyl group in the 3' position of the pseudosaccharide.
References
Molecular Cloning: A Laboratory Manual, Vol 1.
