Kafatos:Minipreps - Revision history

Vaishnavi Ananth: /* BioStream version */

2009-11-19T09:48:03Z

BioStream version

←Older revision		Revision as of 09:48, 19 November 2009
Line 21:		Line 21:
	note: plasmids obtained with this protocol are not clean enough for sequencing<br>		note: plasmids obtained with this protocol are not clean enough for sequencing<br>

-	==~~BioStream~~ version==	+	==BioCoder version==
-	Following is the Kafatos:Minipreps protocol in ~~BioStream~~, a high-level programming language for expressing biology protocols. What you see here is the auto-generated text ouput of the protocol that was coded up in ~~BioStream~~ (see Source code). More information about ~~BioStream~~ can be found on my home page. Feel free to mail me your comments/ suggestions.[[User:Vaishnavi Ananth\|Vaishnavi]]	+	Following is the Kafatos:Minipreps protocol in BioCoder, a high-level programming language for expressing biology protocols. What you see here is the auto-generated text ouput of the protocol that was coded up in BioCoder (see Source code). More information about BioCoder can be found on my home page. Feel free to mail me your comments/ suggestions.[[User:Vaishnavi Ananth\|Vaishnavi]]
	====Text Output====		====Text Output====
	[[Kafatos:Minipreps protocol]]		[[Kafatos:Minipreps protocol]]

Vaishnavi Ananth at 07:04, 20 October 2009

2009-10-20T07:04:37Z

←Older revision		Revision as of 07:04, 20 October 2009
Line 20:		Line 20:

	note: plasmids obtained with this protocol are not clean enough for sequencing<br>		note: plasmids obtained with this protocol are not clean enough for sequencing<br>
		+
		+	==BioStream version==
		+	Following is the Kafatos:Minipreps protocol in BioStream, a high-level programming language for expressing biology protocols. What you see here is the auto-generated text ouput of the protocol that was coded up in BioStream (see Source code). More information about BioStream can be found on my home page. Feel free to mail me your comments/ suggestions.[[User:Vaishnavi Ananth\|Vaishnavi]]
		+	====Text Output====
		+	[[Kafatos:Minipreps protocol]]
		+	====Source Code====
		+	[[Kafatos:Minipreps protocol - source code]]

	[[Category:Protocol]]		[[Category:Protocol]]
	[[Category:in vitro]]		[[Category:in vitro]]
	[[Category:DNA]]		[[Category:DNA]]

Jakob Suckale: categories

2009-05-05T13:01:17Z

Smeister at 11:17, 30 May 2006

2006-05-30T11:17:43Z

←Older revision		Revision as of 11:17, 30 May 2006
Line 7:		Line 7:
	===Day 2===		===Day 2===
	- spin 1.5 ml 2’ @ 8,000 rpm in table top centrifuge<br>		- spin 1.5 ml 2’ @ 8,000 rpm in table top centrifuge<br>
-	- resuspend pellet in 100 l P1 Buffer. Let sit 5’ @ RT<br>	+	- resuspend pellet in 100 μl [[Kafatos:P1\|P1 Buffer]]. Let sit 5’ @ RT<br>
-	- add 200 μl P2 Buffer, mix and let sit no more than 5’ @ RT<br>	+	- add 200 μl [[Kafatos:P2\|P2 Buffer]], mix and let sit no more than 5’ @ RT<br>
-	- add 150 μl ice cold P3 Buffer, mix well and place 5 – 10’ on ice<br>	+	- add 150 μl ice cold [[Kafatos:P3\|P3 Buffer]], mix well and place 5 – 10’ on ice<br>
	- spin 10’ @ max rpm (4˚C if possible)<br>		- spin 10’ @ max rpm (4˚C if possible)<br>
	- transfer supernatant to new tube and add 2 volumes of abs EtOH. Let sit for 10 – 20’ on ice<br>		- transfer supernatant to new tube and add 2 volumes of abs EtOH. Let sit for 10 – 20’ on ice<br>

Smeister at 11:08, 30 May 2006

2006-05-30T11:08:50Z

New page

{{Kafatos/Christophides Lab}}
 

===Day 1===
- inoculate 5 ml sterile LB medium (+ antibiotic ?) with single bacterial colony 
- grow ON @ 37˚C while shaking vigorously 
===Day 2===
- spin 1.5 ml 2’ @ 8,000 rpm in table top centrifuge 
- resuspend pellet in 100 l P1 Buffer. Let sit 5’ @ RT 
- add 200 μl P2 Buffer, mix and let sit no more than 5’ @ RT 
- add 150 μl ice cold P3 Buffer, mix well and place 5 – 10’ on ice 
- spin 10’ @ max rpm (4˚C if possible) 
- transfer supernatant to new tube and add 2 volumes of abs EtOH. Let sit for 10 – 20’ on ice 
- spin 10’ @ max. speed @ 4˚C 
- discard supernatant and wash pellet with 1ml of 70% EtOH 
- spin 5’ @ max speed 
- suck up or decant supernatant, speedvac or dry pellet @ 37˚C (do not overdo it, though, or it will not properly dissolve again) 
- resuspend pellet in 30 μl TE Buffer or ddH20 and check on gel 

note: plasmids obtained with this protocol are not clean enough for sequencing

←Older revision		Revision as of 13:01, 5 May 2009
Line 20:		Line 20:

	note: plasmids obtained with this protocol are not clean enough for sequencing<br>		note: plasmids obtained with this protocol are not clean enough for sequencing<br>
		+
		+	[[Category:Protocol]]
		+	[[Category:in vitro]]
		+	[[Category:DNA]]