Kafatos:10x PBS: Difference between revisions

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'''Add H2O to 1L'''
'''Add H2O to 1L'''
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'''Autoclave for 20 minutes on liquid cycle. Store at RT. '''<BR>
'''Autoclave on liquids cycle. Store at RT.'''<BR>

Revision as of 04:03, 6 March 2006

10x PBS

Combine the following:

80 g NaCl
2 g KCl
14.4 g Na2HPO4
2.4 g KH2PO4
800 mL distilled H2O


Adjust pH to 7.4 with HCl
Add H2O to 1L
Autoclave on liquids cycle. Store at RT.