Julius B. Lucks/Meetings and Notes/01162008 T Ham: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(New page: == Meeting with Tim Ham == * all reagents listed in paper are available ** inducible Fim and Hin ** many RBSs ** diff promoter systems * none in biobrick format, but restriction sites are ...) |
(No difference)
|
Latest revision as of 17:59, 16 January 2008
Meeting with Tim Ham
- all reagents listed in paper are available
- inducible Fim and Hin
- many RBSs
- diff promoter systems
- none in biobrick format, but restriction sites are outside of what is important
- would start off by re-casting into biobricks - then can easily see what can do (take things out, leave things, move around) - test the limiting parameters
- single inversion works
- parts of double inversion don't work - not sure why
- Hin - part of a big family (Gin, etc.) - swappable - all have same core binding site
- Fim - native to E. coli - no other members in this family
- FimB - reversible
- FimE - inverts in one direction
- project idea - synthetic counters
- overlapping inversions like a latch (talk to Josh Gilmore from Keasling lab)
- problem is need at least 4 independent invertases - they don't exist - would have to engineer them so that they don't have cross-talk
- not clear how would screen or select for
- engineering Zn-finger binding domains
- read details of the 1st paper
