Joyce: T-tailing pBSKS: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(New page: Procedure #Obtain about 20 ug of pBSKS #Cut with a blunt cutting restriction enzyme (like EcoRV) #Extract the DNA with 1X phenol and 2X ether #Precipitate the DNA) |
(No difference)
|
Revision as of 09:12, 9 September 2009
- Obtain about 20 ug of pBSKS
- Cut with a blunt cutting restriction enzyme (like EcoRV)
- Extract the DNA with 1X phenol and 2X ether
- Precipitate the DNA