Joyce: T-tailing pBSKS: Difference between revisions
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(New page: Procedure #Obtain about 20 ug of pBSKS #Cut with a blunt cutting restriction enzyme (like EcoRV) #Extract the DNA with 1X phenol and 2X ether #Precipitate the DNA) |
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Revision as of 09:12, 9 September 2009
- Obtain about 20 ug of pBSKS
- Cut with a blunt cutting restriction enzyme (like EcoRV)
- Extract the DNA with 1X phenol and 2X ether
- Precipitate the DNA
