Jonesma Lab:Protocols:digest
From OpenWetWare
Restriction digest
- Determine the concentration of your DNA using the NanoDrop
- Use the ThermoFisher Calculator to determine which buffer to use for your reaction
- Determine the volumes needed to add 5 μg DNA to a 20 μL reaction using the information gathered above. Use dH2O to make up to 20 μL
- Incubate as described by ThermoFisher
- Either store your DNA at -20°C or continue to perform gel electrophoresis to separate your sample