Jhdavis::4x reducing SDS-PAGE loading buffer

4X SDS-PAGE sample loading buffer From OpenWetWare

10 ml 1X

2.0 ml 1M Tris-HCl, pH 6.8 50 mM

0.8 g SDS 2%

4.0 ml 100% glycerol 10%

0.4 ml 14.7 M -mercaptoethanol 1%

1.0 ml 0.5 M EDTA 12.5 mM

8.0 mg bromophenol blue 0.02%

2.6 ml H2O

Use at 1X for loading samples onto SDS-polyacrylamide gels.