Jeff Tabor/chloramphenicol amplification

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Revision as of 14:40, 28 February 2007 by JTabor (talk | contribs) (New page: *Grow a bacterial culture carrying a plasmid to be prepped to mid-log phase *Add 170ug/mL (5x) chloramphenicol to media *Incubate shaking at 37C ~3h *Prep as with standard high copy plasmi...)
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  • Grow a bacterial culture carrying a plasmid to be prepped to mid-log phase
  • Add 170ug/mL (5x) chloramphenicol to media
  • Incubate shaking at 37C ~3h
  • Prep as with standard high copy plasmid
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