Jeff Tabor/AVPR1a R3 amplifification: Difference between revisions
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(New page: 1. Cheek swab into 30uL H20 <br> 2. Colony PCR (Taq) == Cycling parameters == 99 degrees; 10' 72 degrees 94 degrees, 2'<br> 94 degrees, 30"<br> 45 degrees, 30"<br> 72 degrees, 20"<br> ...) |
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Latest revision as of 16:35, 25 November 2008
1. Cheek swab into 30uL H20
2. Colony PCR (Taq)
Cycling parameters
99 degrees; 10'
72 degrees
94 degrees, 2'
94 degrees, 30"
45 degrees, 30"
72 degrees, 20"
[40 cycles]
72 degrees, 5'
