Jasieniuk:Notebook/Apomixis in hybrid Rubus/2010/11/05: Difference between revisions

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(Autocreate 2010/11/05 Entry for Jasieniuk:Notebook/Apomixis_in_hybrid_Rubus)
 
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==Entry title==
==Setup for next round of do-overs==
* Insert content here...
* All parental DNA is now transferred to strip tubes:
 
{| border = "1"
!  !! Strip 1 !! Strip 2 !! Strip 3
|-
| A || 01-01D || 01-06C || 05-02H
|-
| B || 01-02C || 01-06D || 05-03G
|-
| C || 04-02B || 01-06E ||
|-
| D || 05-02G || 01-06G ||
|-
| E || 05-03B || 01-07E ||
|-
| F || 02-10B || 01-10C ||
|-
| G || 02-10C || 01-10G ||
|-
| H || 01-05E || 03-01H ||
|}
 
* Strip tube 1 A-G had been transferred earlier in October
* 01-05E, 01-06D, 01-06E, 01-06G, and 01-07E were diluted from original tubes, because these wells in dilution plate 1 were empty.
* Pipetted DNA onto four PCR plates: parental DNA, new seed plate A, old seed plate 1, and (panel 1 only) leaf (seedling) plate.





Revision as of 14:59, 5 November 2010

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Setup for next round of do-overs

  • All parental DNA is now transferred to strip tubes:
Strip 1 Strip 2 Strip 3
A 01-01D 01-06C 05-02H
B 01-02C 01-06D 05-03G
C 04-02B 01-06E
D 05-02G 01-06G
E 05-03B 01-07E
F 02-10B 01-10C
G 02-10C 01-10G
H 01-05E 03-01H
  • Strip tube 1 A-G had been transferred earlier in October
  • 01-05E, 01-06D, 01-06E, 01-06G, and 01-07E were diluted from original tubes, because these wells in dilution plate 1 were empty.
  • Pipetted DNA onto four PCR plates: parental DNA, new seed plate A, old seed plate 1, and (panel 1 only) leaf (seedling) plate.