Jasieniuk:Notebook/Apomixis in hybrid Rubus/2010/07/19: Difference between revisions

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==Experimental Design==
==Experimental Design==
===List of mother plants===
===List of mother plants===
* Two genotypes of ''R. ursinus'' x ''armeniacus'': are these apomictic or sexual?
* One genotype of ''R. ursinus'' x ''pensilvanicus'': are these apomictic or sexual?
* ''R. ursinus'': expect to see sexual recombination (dioecious)
* ''R. armeniacus'': expect ~90% asexual progeny
* ''R. pensilvanicus'': expect to see mostly asexual progeny
* ''R. ulmifolius'': an extra control; should reproduce sexually
* Another clone that is similar to ''R. armeniacus'': also expect to see ~90% asexual progeny.  This clone is included because it was unexpected that we found a second invasive clone on the West Coast, and so characterizing it in general would be a good idea.
===Microsatellite markers===
===Microsatellite markers===
===Analysis===
===Analysis===

Revision as of 16:55, 19 July 2010

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Experimental Design

List of mother plants

  • Two genotypes of R. ursinus x armeniacus: are these apomictic or sexual?
  • One genotype of R. ursinus x pensilvanicus: are these apomictic or sexual?
  • R. ursinus: expect to see sexual recombination (dioecious)
  • R. armeniacus: expect ~90% asexual progeny
  • R. pensilvanicus: expect to see mostly asexual progeny
  • R. ulmifolius: an extra control; should reproduce sexually
  • Another clone that is similar to R. armeniacus: also expect to see ~90% asexual progeny. This clone is included because it was unexpected that we found a second invasive clone on the West Coast, and so characterizing it in general would be a good idea.

Microsatellite markers

Analysis

References

PCR Run today

Stella, you can put whatever notes you like in here about what you are doing.