Janet B. Matsen:Useful Links

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Revision as of 09:50, 10 July 2012 by Janet B. Matsen (Talk | contribs)
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Useful Links

  • BioNumbers
    • Save time searching for cell facts such as cellular volume or concentration of ATP
  • Finnzyme Primer Dimer Predictor
    • Enter yours here see predicted dimers
  • BitesizeBio
    • They have short but useful articles on lab technique and often include references. I am not a fan of all the advertising.
  • emolecules
    • See who sells compounds you want to buy. Will help you shop around & give more options when your default supplier shows a compound as being back-ordered.
  • BenchFly: free science videos
  • EcoSal  !!subscription required!! (I don't have it at UW)
    • an E. Coli & Salmonella reference website (originally, this info was published in books)
  • RBS strength calculator
    • Recomended by Amanda Smith via Rob Egbert
    • probably accurate within an order of magnitude
    • If you use the forward engineering mode, check your solution in the reverse engineering mode (include ~ 75 bp on either side of the start codon) and make sure it still agrees with it's solution.
    • The sequence matters a lot! High translation is ~___. (If using the Elowitz RBS, the sequence between this part & the ATG matters!

Equipment

Software

  • APE (A Plasmid Editor; free)
  • Mendeley (free)
  • InkScape (free)
Personal tools