Janet B. Matsen:Useful Links

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Useful Links

• BioNumbers
  • Save time searching for cell facts such as cellular volume or concentration of ATP
• Finnzyme Primer Dimer Predictor
  • Enter yours here see predicted dimers
• BitesizeBio
  • They have short but useful articles on lab technique and often include references. I am not a fan of all the advertising.
• emolecules
  • See who sells compounds you want to buy. Will help you shop around & give more options when your default supplier shows a compound as being back-ordered.
• BenchFly: free science videos
  • Sample video: sterile technique
• EcoSal  !!subscription required!! (I don't have it at UW)
  • an E. Coli & Salmonella reference website (originally, this info was published in books)
• RBS strength calculator
  • Recomended by Amanda Smith via Rob Egbert
  • probably accurate within an order of magnitude
  • If you use the forward engineering mode, check your solution in the reverse engineering mode (include ~ 75 bp on either side of the start codon) and make sure it still agrees with it's solution.
  • The sequence matters a lot! High translation is ~___. (If using the Elowitz RBS, the sequence between this part & the ATG matters!

Equipment

• NanoDrop basics (nucleic acid concentration measurements)

Software

• APE (A Plasmid Editor; free)
• Mendeley (free)
• InkScape (free)
  • Can get beautiful color paletes from ColorBrewer