Janet B. Matsen:Useful Links

(Difference between revisions)

Revision as of 14:16, 2 July 2012

Back to Janet

BioNumbers
- Save time searching for cell facts such as cellular volume or concentration of ATP
Finnzyme Primer Dimer Predictor
- Enter yours here see predicted dimers
BitesizeBio
- They have short but useful articles on lab technique and often include references. I am not a fan of all the advertising.
emolecules
- See who sells compounds you want to buy. Will help you shop around & give more options when your default supplier shows a compound as being back-ordered.
BenchFly: free science videos
- Sample video: sterile technique
EcoSal !!subscription required!! (I don't have it at UW)
- an E. Coli & Salmonella reference website (originally, this info was published in books)
RBS strength calculator
- Recomended by Amanda Smith via Rob Egbert
- The sequence matters a lot! High translation is ~___. (If using the Elowitz RBS, the sequence between this part & the ATG matters!

@@ Line 15: / Line 15: @@
 ** an E. Coli & Salmonella reference website (originally, this info was published in books)
 *[https://salis.psu.edu/software/reverse RBS strength calculator]
-** Recomended by Amanda Smith  (she says the Klavins lab uses a different one)
+** Recomended by Amanda Smith via Rob Egbert
-**The sequence matters a lot!  (If using the Elowitz RBS, the sequence between this part & the ATG matters!
+**The sequence matters a lot!  High translation is ~___.  (If using the Elowitz RBS, the sequence between this part & the ATG matters!
-** Elowitz RBS + BioBrick Scar site + ATG --> ~ 14000 units of transcription, I think.
 ==Equipment==