Janet B. Matsen:Closed Lab Questions

From OpenWetWare

Revision as of 00:31, 24 February 2012 by Janet B. Matsen (Talk | contribs)
Jump to: navigation, search

Back to Janet

I define "closed" as "not bothering me incessantly." One can always learn more but we have to stop at a point otherwise we would never move forward with our research!

If you have any input/corrections/references please drop [User:Janet B. Matsen|me] a line.

Contents

Colony PCR

  • Q: The gel is dark where I loaded it -- what does this mean?
    • A: It probably means you overloaded the PCR with too many cells.

Primers/Oligos

Should I pay extra to further purify large bp oligos?

  • Usually no. The sequencing companies just want your money. Do sequence your creations to confirm that you made what you expected and remember you can always screen a new colony if the first one looks bad.

My primers are yellow!

  • Don't worry -- this is normal!
    normal oligos have a yellow color that is visible with large synthesis scales
    normal oligos have a yellow color that is visible with large synthesis scales
Personal tools