Janet B. Matsen:Closed Lab Questions

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===My primers are yellow!===
===My primers are yellow!===
*Don't worry -- this is normal!  [[Image:Yellow_primers.jpg|thumb|normal oligos have a yellow color that is visible with large synthesis scales]]
*Don't worry -- this is normal!  [[Image:Yellow_primers.jpg|thumb|normal oligos have a yellow color that is visible with large synthesis scales]]
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==BioBricks==
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=== The parts in the registry have sequence that don't include the prefix & suffix... do they come with it? ===
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*Yes, they come with a prefix/suffix. You can find which BioBrick method a part is compatable with under the part's "Assembly Compatibility" listing.  Most are RFC 10 (http://partsregistry.org/partsdb/scars.cgi), the standard format.

Revision as of 09:18, 28 February 2012

Back to Janet

I define "closed" as "not bothering me incessantly." One can always learn more but we have to stop at a point otherwise we would never move forward with our research!

If you have any input/corrections/references please drop [User:Janet B. Matsen|me] a line.

Contents

Colony PCR

  • Q: The gel is dark where I loaded it -- what does this mean?
    • A: It probably means you overloaded the PCR with too many cells.

Primers/Oligos

Should I pay extra to further purify large bp oligos?

  • Usually no. The sequencing companies just want your money. Do sequence your creations to confirm that you made what you expected and remember you can always screen a new colony if the first one looks bad.

My primers are yellow!

  • Don't worry -- this is normal!
    normal oligos have a yellow color that is visible with large synthesis scales
    normal oligos have a yellow color that is visible with large synthesis scales

BioBricks

The parts in the registry have sequence that don't include the prefix & suffix... do they come with it?

  • Yes, they come with a prefix/suffix. You can find which BioBrick method a part is compatable with under the part's "Assembly Compatibility" listing. Most are RFC 10 (http://partsregistry.org/partsdb/scars.cgi), the standard format.
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