Janet B. Matsen:Closed Lab Questions

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(My primers are yellow!)
Line 9: Line 9:
**A: It probably means you overloaded the PCR with too many cells.
**A: It probably means you overloaded the PCR with too many cells.
-
==My primers are yellow!==
+
==Primers/Oligos==
 +
===Should I pay extra to further purify large bp oligos?===
 +
*Usually no.  The sequencing companies just want your money.  Do sequence your creations to confirm that you made what you expected and remember you can always screen a new colony if the first one looks bad.
 +
===My primers are yellow!===
*Don't worry -- this is normal!  [[Image:Yellow_primers.jpg|thumb|normal oligos have a yellow color that is visible with large synthesis scales]]
*Don't worry -- this is normal!  [[Image:Yellow_primers.jpg|thumb|normal oligos have a yellow color that is visible with large synthesis scales]]

Revision as of 00:31, 24 February 2012

Back to Janet

I define "closed" as "not bothering me incessantly." One can always learn more but we have to stop at a point otherwise we would never move forward with our research!

If you have any input/corrections/references please drop [User:Janet B. Matsen|me] a line.

Contents

Colony PCR

  • Q: The gel is dark where I loaded it -- what does this mean?
    • A: It probably means you overloaded the PCR with too many cells.

Primers/Oligos

Should I pay extra to further purify large bp oligos?

  • Usually no. The sequencing companies just want your money. Do sequence your creations to confirm that you made what you expected and remember you can always screen a new colony if the first one looks bad.

My primers are yellow!

  • Don't worry -- this is normal!
    normal oligos have a yellow color that is visible with large synthesis scales
    normal oligos have a yellow color that is visible with large synthesis scales
Personal tools