James P. McDonald Week 3: Difference between revisions
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**The ammonia concentrations were varied to observe its effects on gene expression and enzyme activities. | **The ammonia concentrations were varied to observe its effects on gene expression and enzyme activities. | ||
*The main result of the study was that nitrogen metabolism is dependent on ammonia concentration, not its flux. | *The main result of the study was that nitrogen metabolism is dependent on ammonia concentration, not its flux. | ||
*Ammonia is the prefferred growth source of ''Saccharomyces cerevisiae'' as it results in faster growth. | *Ammonia is the prefferred growth source of ''Saccharomyces cerevisiae'' as it results in faster growth. |
Revision as of 19:07, 30 January 2013
Biological Terms
- Permease: "General term for a membrane protein that increases the permeability of the plasma membrane to a particular molecule, by a process not requiring metabolic energy." [[1]]
- Isomerase: "An enzyme that converts molecules into their positional isomers." [[2]]
- Oligonucleotides: "Polymers made up of a few (2-20) nucleotides. In molecular genetics, they refer to a short sequence synthesised to match a region where a mutation is known to occur, and then used as a probe (oligonucleotide probes)." [[3]]
- Dehydrogenase: "Enzyme that oxidizes a substrate by transferring hydrogen to an acceptor that is either NAD/NADP or a flavin enzyme. An enzyme that is used to remove hydrogen from its substrate, which is used in the cytochrome (hydrogen carrier) system in respiration to produce a net gain of ATP." [[4]]
- Synthetase: "Enzymes of class 6 in the e classification, catalyse synthesis of molecules, their activity being coupled to the breakdown of a nucleotide triphosphate." [[5]]
- Biosynthetic: "Relating to or produced by biosynthesis." [[6]]
- Glutamate: "Major fast excitatory neurotransmitter in the mammalian central nervous system." [[7]]
- Glutamine: "A crystalline amino acid occurring in proteins; important in protein metabolism. One of the 20 amino acids that are commonly found in proteins." [[8]]
- GAP1: "General amino acid permease, a gene found in Saccharomyces cerevisiae." [[9]]
- PUT4: "Proline permease, a gene found in Saccharomyces cerevisiae." [[10]]
Outline
Introduction/Background
The Concentration of Ammonia Regulates Nitrogen Metabolism in Saccharomyces cerevisiae
- Saccharomyces cerevisiae was grown in various ammonia concentrations and the effects on the growth was observed.
- A single dilution rate was using with a range of different ammonia concentrations.
- The ammonia concentrations were varied to observe its effects on gene expression and enzyme activities.
- The main result of the study was that nitrogen metabolism is dependent on ammonia concentration, not its flux.
- Ammonia is the prefferred growth source of Saccharomyces cerevisiae as it results in faster growth.
- Nitrogen metabolism is regulated by gene expression and enzyme activity.
- Previous research seems to show that ammonia concentration itself is the most important factor in nitrogen metabolism.
- But, in these previous studies the cultures have differed in ammonium flux, leaving flux as the possible key factor.
- This experiment uses cultures with the same level flux, only the ammonium concentrations fed in are different.
Methods
Physiological Parameters
Northern Analyses
Enzyme Activities
Class Links
- James P. McDonald
- BIOL 398 Home Page
- Week 1 Assignment
- Week 2 Assignment
- Week 3 Assignment
- Week 4 Assignment
- Week 5 Assignment
- Week 6 Assignment
- Week 8 Assignment
- Week 9 Assignment
- Week 11 Assignment
- Week 12 Assignment
- Week 13 Assignment
- Week 14 Assignment
Journal Entries and Assignments
- Class Journal Week 1
- Class Journal Week 2
- Class Journal Week 3
- Class Journal Week 4
- Class Journal Week 5
- Class Journal Week 6
- Class Journal Week 8
- Class Journal Week 9
- Class Journal Week 11
- Class Journal Week 12
- Class Journal Week 13
- Class Journal Week 14
- James P. McDonald Week 2
- James P. McDonald Week 3
- James P. McDonald Week 4
- James P. McDonald Week 5
- James P. McDonald Week 6
- James P. McDonald Week 8
- James P. McDonald Week 9
- James P. McDonald Week 11
- James P. McDonald Week 12
- James P. McDonald Week 13
- James P. McDonald Week 14