James P. McDonald Week 3

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#'''''Glutamate''''': "Major fast excitatory neurotransmitter in the mammalian central nervous system." [[http://www.biology-online.org/dictionary/Glutamate]]
#'''''Glutamate''''': "Major fast excitatory neurotransmitter in the mammalian central nervous system." [[http://www.biology-online.org/dictionary/Glutamate]]
#'''''Glutamine''''': "A crystalline amino acid occurring in proteins; important in protein metabolism. One of the 20 amino acids that are commonly found in proteins." [[http://www.biology-online.org/dictionary/Glutamine]]
#'''''Glutamine''''': "A crystalline amino acid occurring in proteins; important in protein metabolism. One of the 20 amino acids that are commonly found in proteins." [[http://www.biology-online.org/dictionary/Glutamine]]
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#'''''GAP1''''': "General amino acid permease, a gene found in <i>Saccharomyces cerevisiae</i>." [[http://amigo.geneontology.org/cgi-bin/amigo/gp-details.cgi?gp=SGD:S000001747&session_id=9053amigo1359594666]]
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#'''''GAP1''''': "General amino acid permease, a gene found in ''Saccharomyces cerevisiae''." [[http://amigo.geneontology.org/cgi-bin/amigo/gp-details.cgi?gp=SGD:S000001747&session_id=9053amigo1359594666]]
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#'''''PUT4''''': "Proline permease, a gene found in <i>Saccharomyces cerevisiae</i>." [[http://amigo.geneontology.org/cgi-bin/amigo/gp-details.cgi?gp=SGD:S000005875&session_id=9053amigo1359594666]]
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#'''''PUT4''''': "Proline permease, a gene found in ''Saccharomyces cerevisiae''." [[http://amigo.geneontology.org/cgi-bin/amigo/gp-details.cgi?gp=SGD:S000005875&session_id=9053amigo1359594666]]
==Outline==
==Outline==
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===Introduction===
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*''Saccharomyces cerevisiae'' was grown in various ammonia concentrations and the effects on the growth was observed.
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**A single dilution rate was using with a range of different ammonia concentrations.
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**The ammonia concentrations were varied to observe its effects on gene expression and enzyme activities.
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*The main result of the study was that nitrogen metabolism is dependent on ammonia concentration, not its flux.
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===Methods===
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====Physiological Parameters====
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====Northern Analyses====
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====Enzyme Activities====
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{{James P. McDonald}}
{{James P. McDonald}}
[[Category:BIOL398-03/S13]]
[[Category:BIOL398-03/S13]]

Revision as of 20:55, 30 January 2013

Contents

Biological Terms

  1. Permease: "General term for a membrane protein that increases the permeability of the plasma membrane to a particular molecule, by a process not requiring metabolic energy." [[1]]
  2. Isomerase: "An enzyme that converts molecules into their positional isomers." [[2]]
  3. Oligonucleotides: "Polymers made up of a few (2-20) nucleotides. In molecular genetics, they refer to a short sequence synthesised to match a region where a mutation is known to occur, and then used as a probe (oligonucleotide probes)." [[3]]
  4. Dehydrogenase: "Enzyme that oxidizes a substrate by transferring hydrogen to an acceptor that is either NAD/NADP or a flavin enzyme. An enzyme that is used to remove hydrogen from its substrate, which is used in the cytochrome (hydrogen carrier) system in respiration to produce a net gain of ATP." [[4]]
  5. Synthetase: "Enzymes of class 6 in the e classification, catalyse synthesis of molecules, their activity being coupled to the breakdown of a nucleotide triphosphate." [[5]]
  6. Biosynthetic: "Relating to or produced by biosynthesis." [[6]]
  7. Glutamate: "Major fast excitatory neurotransmitter in the mammalian central nervous system." [[7]]
  8. Glutamine: "A crystalline amino acid occurring in proteins; important in protein metabolism. One of the 20 amino acids that are commonly found in proteins." [[8]]
  9. GAP1: "General amino acid permease, a gene found in Saccharomyces cerevisiae." [[9]]
  10. PUT4: "Proline permease, a gene found in Saccharomyces cerevisiae." [[10]]

Outline

Introduction

  • Saccharomyces cerevisiae was grown in various ammonia concentrations and the effects on the growth was observed.
    • A single dilution rate was using with a range of different ammonia concentrations.
    • The ammonia concentrations were varied to observe its effects on gene expression and enzyme activities.
  • The main result of the study was that nitrogen metabolism is dependent on ammonia concentration, not its flux.

Methods

Physiological Parameters

Northern Analyses

Enzyme Activities

Class Links

Journal Entries and Assignments

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