Jacobs:Back Door

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Useful Links / Meetings

• Chemtracker
• ORS
• ASME SBC
• ASBMR
• BMES

Protocols

Research Protocols

• General
  • Printers
  • Safety
  • Autoclave
  • Leica MP Microscope
• Cells
  • Culture
    • Culture of MLO-Y4s
    • Culture of IMCDs
    • Culture of MDCKs
    • Culture of 3T3 Fibroblasts
    • Cell Culture numbers
    • Troubleshooting Cell Culture
    • Freezing and Thawing Cells
  • Transfection
    • siRNA transfection
    • Neon Transfection
    • Transfecting NIH 3T3s with GFP-Actin
  • Coating with Fibronectin
  • Isolation of Primary Bone Cells
  • Total Protein Isolation Using RIPA Lysis Buffer
  • Total RNA Isolation using Tri Reagent
• Flow
  • OFF (Large Chambers)
  • Flow chamber calibration
  • Confocal imaging of primary cilia under flow

• Immunocytochemistry
  • Immunostaining for TRPV4 and PC
  • Preparation of primary osteocytes and MDCK cells for immunostaining
  • Actin Staining
  • Tubulin Immunostaining
• Assays
  • BCA Total Protein Content
  • Real-Time RT qPCR
  • Gene expression of osteocyte markers
  • Alkaline Phosphatase
• Gels & blots
  • Protein
    • NuPAGE Electrophoresis and Western Blotting for Proteins
    • Protein Gel Staining Using Coomassie Blue Protein Stain
    • Protein SDS-PAGE and Coomassie Staining of Polyacrylamide Gel for Protein
  • DNA & RNA
    • Polymerase Chain Reaction (PCR) Set-up and DNA Agarose Gel
    • Agarose Formaldehyde Gel Preparation for RNA Electrophoresis (1)
    • Agarose Formaldehyde Gel Preparation for RNA Electrophoresis (2)
    • 10 bp DNA Ladder
• Mice
  • Separating and Tagging of Mice
  • Genotyping by qPCR
  • Genotyping by agarose gel
  • Loading Mice
  • In vivo microCT scanning protocol for live mice

Teaching Protocols

• Pipetting Exercise
• Adipocyte Differentiation
• Oil Red O Staining
• RNA Agarose Gel
• Collagen Gel Contraction
• Collagen Gel Contraction 2
• Total Protein Isolation using RIPA buffer
• Total Protein Isolation using RIPA buffer 2
• RNA Sample Loading Buffer

Miscellaneous

• Photos:Jacobs photos
• Library
• Movies