Jacobs:Back Door: Difference between revisions

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**[[Jacobs: Cell Culture of IMCD Kidney cells | Culture of IMCDs]]
**[[Jacobs: Cell Culture of IMCD Kidney cells | Culture of IMCDs]]
**[[Jacobs:Protocol Cell Culture of MDCK cells |Culture of MDCKs]]
**[[Jacobs:Protocol Cell Culture of MDCK cells |Culture of MDCKs]]
**[[Jacobs:Protocol Subculturing 3T3 Fibroblasts|Culture of 3T3 Fibroblasts]]
**[[Jacobs: Troubleshooting Cell Culture| Troubleshooting Cell Culture]]
**[[Jacobs: Troubleshooting Cell Culture| Troubleshooting Cell Culture]]
**[[Jacobs:Cell Culture Numbers| Cell Culture numbers]]
**[[Jacobs:Cell Culture Numbers| Cell Culture numbers]]
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**[[Jacobs:Protocol Transfecting NIH 3T3s with GFP-Actin|Transfecting NIH 3T3s with GFP-Actin]]
**[[Jacobs:Protocol Transfecting NIH 3T3s with GFP-Actin|Transfecting NIH 3T3s with GFP-Actin]]
**[[Jacobs:Protocol Total Protein Isolation Using RIPA Lysis Buffer|Total Protein Isolation Using RIPA Lysis Buffer]]
**[[Jacobs:Protocol Total Protein Isolation Using RIPA Lysis Buffer|Total Protein Isolation Using RIPA Lysis Buffer]]
**[[Jacobs:Protocol Total RNA Isolation using Tri Reagent|Total RNA Isolation using Tri Reagent]]
*Flow
*Flow
**[[Jacobs:Protocol OFF (Large Chambers)|OFF (Large Chambers)]]
**[[Jacobs:Protocol OFF (Large Chambers)|OFF (Large Chambers)]]
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**[[Jacobs:Tubulin Immunostaining Protocol|Tubulin Immunostaining]]
**[[Jacobs:Tubulin Immunostaining Protocol|Tubulin Immunostaining]]
*Assays
*Assays
*[[Jacobs:Protocol BCA_Total_Protein|BCA Total Protein Content]]
**[[Jacobs:Protocol Real-Time PCR|Real-Time RT qPCR]]
**[[Jacobs:Protocol Osteocyte Markers|Gene expression of osteocyte markers]]
**[[Jacobs:Protocol Osteocyte Markers|Gene expression of osteocyte markers]]
**[[Jacobs:Protocol Alkaline Phosphatase Assay | Alkaline Phosphatase]]
**[[Jacobs:Protocol Alkaline Phosphatase Assay | Alkaline Phosphatase]]
*Gels & blots
*Gels & blots
*[[Jacobs:Protocol Polymerase Chain Reaction (PCR) Set-up and DNA Agarose Gel|Polymerase Chain Reaction (PCR) Set-up and DNA Agarose Gel]]
**Protein
*[[Jacobs:Protocol Protein Gel Staining Using Coomassie Blue Protein Stain|Protein Gel Staining Using Coomassie Blue Protein Stain]]
***[[Jacobs:Protocol NuPAGE Electrophoresis and Western Blotting for Proteins|NuPAGE Electrophoresis and Western Blotting for Proteins]]
*[[Jacobs:Protocol Protein Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) and Coomassie Staining of Polyacrylamide Gel for Protein|Protein Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE)and Coomassie Staining of Polyacrylamide Gel for Protein]]
***[[Jacobs:Protocol Protein Gel Staining Using Coomassie Blue Protein Stain|Protein Gel Staining Using Coomassie Blue Protein Stain]]
*[[Jacobs:Protocol Agarose Formaldehyde Gel Preparation for RNA and Electrophoresis|Agarose Formaldehyde Gel Preparation for RNA Electrophoresis (1)]]
***[[Jacobs:Protocol Protein Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) and Coomassie Staining of Polyacrylamide Gel for Protein|Protein SDS-PAGE and Coomassie Staining of Polyacrylamide Gel for Protein]]
*[[Jacobs:Protocol Agarose Formaldehyde Gel Preparation for RNA Electrophoresis|Agarose Formaldehyde Gel Preparation for RNA Electrophoresis (2)]]
**DNA & RNA
***[[Jacobs:Protocol Polymerase Chain Reaction (PCR) Set-up and DNA Agarose Gel|Polymerase Chain Reaction (PCR) Set-up and DNA Agarose Gel]]
***[[Jacobs:Protocol Agarose Formaldehyde Gel Preparation for RNA and Electrophoresis|Agarose Formaldehyde Gel Preparation for RNA Electrophoresis (1)]]
***[[Jacobs:Protocol Agarose Formaldehyde Gel Preparation for RNA Electrophoresis|Agarose Formaldehyde Gel Preparation for RNA Electrophoresis (2)]]
*[[Jacobs:Protocol 10 bp DNA Ladder|10 bp DNA Ladder]]
*[[Jacobs:Protocol 10 bp DNA Ladder|10 bp DNA Ladder]]
* Mouse work
* Mouse work
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===Teaching Protocols===
===Teaching Protocols===
*[[Jacobs:Protocol BCA_Total_Protein|BCA Total Protein]]
*[[Jacobs:Protocol Adipocyte Differentiation|Adipocyte Differentiation]]
*[[Jacobs:Protocol Adipocyte Differentiation|Adipocyte Differentiation]]
*[[Jacobs:Protocol Oil Red O Staining|Oil Red O Staining]]
*[[Jacobs:Protocol Oil Red O Staining|Oil Red O Staining]]
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*[[Jacobs:Protocol Collagen Gel Contraction|Collagen Gel Contraction]]
*[[Jacobs:Protocol Collagen Gel Contraction|Collagen Gel Contraction]]
*[[Jacobs:Protocol Collagen Gel Contraction 2|Collagen Gel Contraction 2]]
*[[Jacobs:Protocol Collagen Gel Contraction 2|Collagen Gel Contraction 2]]
*[[Jacobs:Protocol Real-Time PCR|Real-Time PCR]]
*[[Jacobs:Protocol Pipetting Exercise|Pipetting Exercise]]
*[[Jacobs:Protocol Pipetting Exercise|Pipetting Exercise]]
*[[Jacobs:Protocol Total Protein Isolation using RIPA buffer|Total Protein Isolation using RIPA buffer]]
*[[Jacobs:Protocol Total Protein Isolation using RIPA buffer|Total Protein Isolation using RIPA buffer]]
*[[Jacobs:Protocol Total Protein Isolation using RIPA buffer 2|Total Protein Isolation using RIPA buffer 2]]
*[[Jacobs:Protocol Total Protein Isolation using RIPA buffer 2|Total Protein Isolation using RIPA buffer 2]]
*[[Jacobs:Protocol RNA Sample Loading Buffer|RNA Sample Loading Buffer]]
*[[Jacobs:Protocol RNA Sample Loading Buffer|RNA Sample Loading Buffer]]
*[[Jacobs:Protocol Total RNA Isolation using Tri Reagent|Total RNA Isolation using Tri Reagent]]
*[[Jacobs:Protocol Subculturing 3T3 Fibroblasts|Subculturing 3T3 Fibroblasts]]
*[[Jacobs:Protocol NuPAGE Electrophoresis and Western Blotting for Proteins|NuPAGE Electrophoresis and Western Blotting for Proteins]]


==Miscellaneous==
==Miscellaneous==

Revision as of 12:20, 2 December 2014



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