Jacobs:Back Door: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
An M. Nguyen (talk | contribs) No edit summary |
An M. Nguyen (talk | contribs) |
||
| Line 16: | Line 16: | ||
**[[Jacobs:Protocol Safety|Safety]] | **[[Jacobs:Protocol Safety|Safety]] | ||
**[[Jacobs:Protocol Autoclave | Autoclave]] | **[[Jacobs:Protocol Autoclave | Autoclave]] | ||
**[[Jacobs: Leica MP Microscope Protocol| Leica MP Microscope]] | |||
*Cell culture | *Cell culture | ||
**[[Jacobs:Protocol Freezing and Thawing Cells|Freezing and Thawing Cells]] | **[[Jacobs:Protocol Freezing and Thawing Cells|Freezing and Thawing Cells]] | ||
**[[Jacobs: Cell Culture of MLOY-4 Osteocyte Cells]] | **[[Jacobs: Cell Culture of MLOY-4 Osteocyte Cells | Culture of MLO-Y4s]] | ||
**[[Jacobs: Cell Culture of IMCD Kidney cells]] | **[[Jacobs: Cell Culture of IMCD Kidney cells | Culture of IMCDs]] | ||
**[[Jacobs:Protocol Cell Culture of MDCK cells | Cell Culture | **[[Jacobs:Protocol Cell Culture of MDCK cells |Culture of MDCKs]] | ||
**[[Jacobs: Troubleshooting Cell Culture| Troubleshooting Cell Culture]] | |||
**[[Jacobs:Cell Culture Numbers| Cell Culture numbers]] | **[[Jacobs:Cell Culture Numbers| Cell Culture numbers]] | ||
**[[Jacobs:Protocol Coating Slides with Fibronectin | Coating Slides with Fibronectin]] | **[[Jacobs:Protocol Coating Slides with Fibronectin | Coating Slides with Fibronectin]] | ||
**[[Jacobs: Isolation of Primary Bone Cells| Isolation of Primary Bone Cells]] | **[[Jacobs: Isolation of Primary Bone Cells| Isolation of Primary Bone Cells]] | ||
| Line 32: | Line 33: | ||
**[[Jacobs:Protocol OFF (Large Chambers)|OFF (Large Chambers)]] | **[[Jacobs:Protocol OFF (Large Chambers)|OFF (Large Chambers)]] | ||
**[[Jacobs:Protocol Flow chamber calibration|Flow chamber calibration]] | **[[Jacobs:Protocol Flow chamber calibration|Flow chamber calibration]] | ||
**[[Jacobs:Confocal Imaging of Flow| Confocal | **[[Jacobs:Confocal Imaging of Flow| Confocal imaging of primary cilia under flow]] | ||
*Immunocytochemistry | *Immunocytochemistry | ||
**[[Jacobs: Protocol Immunostaining for TRPV4 and primary cilia | Immunostaining for TRPV4 and PC]] | **[[Jacobs: Protocol Immunostaining for TRPV4 and primary cilia | Immunostaining for TRPV4 and PC]] | ||
**[[Jacobs: Protocol Preparation of primary osteocytes and MDCK cells for immunostaining | Preparation of primary osteocytes and MDCK cells for immunostaining]] | **[[Jacobs: Protocol Preparation of primary osteocytes and MDCK cells for immunostaining | Preparation of primary osteocytes and MDCK cells for immunostaining]] | ||
**[[Jacobs:Protocol Actin Staining|Actin Staining]] | **[[Jacobs:Protocol Actin Staining|Actin Staining]] | ||
**[[Jacobs:Tubulin Immunostaining Protocol|Tubulin Immunostaining Protocol]] | **[[Jacobs:Tubulin Immunostaining Protocol|Tubulin Immunostaining]] | ||
*Assays | |||
**[[Jacobs:Protocol Osteocyte Markers|Gene expression of osteocyte markers]] | |||
**[[Jacobs:Protocol Alkaline Phosphatase Assay | Alkaline Phosphatase]] | |||
*Gels & blots | *Gels & blots | ||
*[[Jacobs:Protocol Polymerase Chain Reaction (PCR) Set-up and DNA Agarose Gel|Polymerase Chain Reaction (PCR) Set-up and DNA Agarose Gel]] | *[[Jacobs:Protocol Polymerase Chain Reaction (PCR) Set-up and DNA Agarose Gel|Polymerase Chain Reaction (PCR) Set-up and DNA Agarose Gel]] | ||
*[[Jacobs:Protocol Protein Gel Staining Using Coomassie Blue Protein Stain|Protein Gel Staining Using Coomassie Blue Protein Stain]] | *[[Jacobs:Protocol Protein Gel Staining Using Coomassie Blue Protein Stain|Protein Gel Staining Using Coomassie Blue Protein Stain]] | ||
*[[Jacobs:Protocol Protein Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) and Coomassie Staining of Polyacrylamide Gel for Protein|Protein Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE)and Coomassie Staining of Polyacrylamide Gel for Protein]] | *[[Jacobs:Protocol Protein Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) and Coomassie Staining of Polyacrylamide Gel for Protein|Protein Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE)and Coomassie Staining of Polyacrylamide Gel for Protein]] | ||
*[[Jacobs:Protocol Agarose Formaldehyde Gel Preparation for RNA Electrophoresis|Agarose Formaldehyde Gel Preparation for RNA Electrophoresis]] | *[[Jacobs:Protocol Agarose Formaldehyde Gel Preparation for RNA and Electrophoresis|Agarose Formaldehyde Gel Preparation for RNA Electrophoresis (1)]] | ||
*[[Jacobs:Protocol Agarose Formaldehyde Gel Preparation for RNA | *[[Jacobs:Protocol Agarose Formaldehyde Gel Preparation for RNA Electrophoresis|Agarose Formaldehyde Gel Preparation for RNA Electrophoresis (2)]] | ||
*[[Jacobs:Protocol 10 bp DNA Ladder|10 bp DNA Ladder]] | *[[Jacobs:Protocol 10 bp DNA Ladder|10 bp DNA Ladder]] | ||
* Mouse work | * Mouse work | ||
| Line 51: | Line 54: | ||
**[[Jacobs:Protocol Loading Mice|Loading Mice]] | **[[Jacobs:Protocol Loading Mice|Loading Mice]] | ||
**[[Jacobs: Protocol In vivo microCT scanning protocol for live mice| In vivo microCT scanning protocol for live mice]] | **[[Jacobs: Protocol In vivo microCT scanning protocol for live mice| In vivo microCT scanning protocol for live mice]] | ||
===Teaching Protocols=== | ===Teaching Protocols=== | ||
Revision as of 12:16, 2 December 2014
Useful Links / Meetings
Protocols
Research Protocols
- General
- Cell culture
- Freezing and Thawing Cells
- Culture of MLO-Y4s
- Culture of IMCDs
- Culture of MDCKs
- Troubleshooting Cell Culture
- Cell Culture numbers
- Coating Slides with Fibronectin
- Isolation of Primary Bone Cells
- siRNA transfection
- Neon Transfection
- Transfecting NIH 3T3s with GFP-Actin
- Total Protein Isolation Using RIPA Lysis Buffer
- Flow
- Immunocytochemistry
- Assays
- Gels & blots
- Polymerase Chain Reaction (PCR) Set-up and DNA Agarose Gel
- Protein Gel Staining Using Coomassie Blue Protein Stain
- Protein Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE)and Coomassie Staining of Polyacrylamide Gel for Protein
- Agarose Formaldehyde Gel Preparation for RNA Electrophoresis (1)
- Agarose Formaldehyde Gel Preparation for RNA Electrophoresis (2)
- 10 bp DNA Ladder
- Mouse work
Teaching Protocols
- BCA Total Protein
- Adipocyte Differentiation
- Oil Red O Staining
- RNA Agarose Gel
- Collagen Gel Contraction
- Collagen Gel Contraction 2
