JCAOligoTutorial1b-TmClarification: Difference between revisions
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JCAnderson (talk | contribs) (New page: === How to analyze the 'secondary structure' of your oligos === When designing oligos, there is some ambiguity about whether you want a high or a low Tm, and exactly what is meant by Tm. ...) |
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Revision as of 17:39, 24 January 2012
How to analyze the 'secondary structure' of your oligos
When designing oligos, there is some ambiguity about whether you want a high or a low Tm, and exactly what is meant by Tm. First, let's disambiguate the term Tm, because there are 2 equally important but different Tm values for a particular DNA sequence.
