Inhibition of Mutation and Combating of the Evolution of Antibiotic Resistance (Cirz et al)
The authors show that the interfering with the protease LexA renders pathogenic E.Coli unable to evolve resistance to a couple antibiotics. They show that lexA cleavage is induced during RecBC-mediated repair of DNA damage and that this results in the derepression of the SOS-regulated polymersaes Pol II, Pol IV, and Pol V which collaborate to induce (resistance confering) mutations.
Cells have a variety of methods to up-regulate mutations in response to stress. (see also references [7-15] in this paper). Knocking down these mechanisms may be a method for increasing the genetic stability of our devices.