IndieBiotech:Back Door: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
m (New page: Here are some things I/We use at Indie Biotech. It's mostly protocols. == E.coli Transformation == By PEG/Mg. Brands verified to work: Miralax and generic "Epsom Salts". == E.coli Minip...) |
(Adding quick discussion of protocols, formatting for lab pages.) |
||
| Line 1: | Line 1: | ||
{{IndieBiotech}} | |||
Here are some things I/We use at Indie Biotech. It's mostly protocols. | Here are some things I/We use at Indie Biotech. It's mostly protocols. | ||
Revision as of 09:21, 18 October 2011
Home - Contact - Internal - Lab Members - Publications - Research - Talks
Here are some things I/We use at Indie Biotech. It's mostly protocols.
E.coli Transformation
By PEG/Mg. Brands verified to work: Miralax and generic "Epsom Salts".
E.coli Miniprep
Have used the Miniprep kit from NBSbio.co.uk successfully, will soon test DIY version with and without columns.
Bacillus subtilis Transformation
Still testing protocols. Candidates:
- Germination of spores in presence of DNA - Growing cells in minimal media to stationary, washing, adding DNA - Adding spores to culture of plasmid-containing E.coli, boiling for 5 minutes, spreading
Bacillus subtilis Miniprep
Not yet tested; presumed as above but with prior lysozyme treatment.
