Image:Homologous Recombination Deletion method one.png
Size of this preview: 388 × 599 pixels
Full resolution (1,184 × 1,829 pixel, file size: 229 KB, MIME type: image/png)
|(5 intermediate revisions not shown.)|
|Line 1:||Line 1:|
Linear sequences of DNA were created using PCR from synthetic primers (the red stretch of DNA). Regions A, B and C inside the target region inside the genome (green) and inside the linear PCR sequence (red) are homologous regions. Cm^R codes for chloramphenicol resistance, S indicates an I-ScsI cleavage site.
The DNA is electroporated into the cell, then a plasmid is inserted coding for recombinase elements used for recombination. Cells that succeed in recombination were isolated.
Another plasmid coding for I-SceI meganuclease was inserted into the cell to remove the Cm^R gene. Then, a version of Rec-A mediated DSB recombinational repair was used to ligate the pieces together.
Click on a date/time to view the file as it appeared at that time.
|current||22:33, 10 February 2013||1,184×1,829 (229 KB)||Evan J. Weaver (Talk | contribs)|
- Search for duplicate files
- Edit this file using an external application
See the setup instructions for more information.