File:DSCF0907.JPG
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To explore various methods of fluorescent detection of Calcium, we either added Fluo-3 AM or transfected inverse-pericam into mouse embryonic stem cells, then treated some cells with histamine and others with cyproheptadine hydrochloride (Calcium mobilizing agents). Here is shown MES cells + Fluo-3 AM + 0.1 uM cyproheptadine hydrochloride @ 10x magnification. We see increased levels of Calcium as a result of adding cyproheptadine (untreated MES cells + Fluo-3 AM show significantly less fluorescence).
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 11:52, 15 March 2007 | | 2,400 × 1,800 (779 KB) | Nzimm (talk | contribs) | To explore various methods of fluorescent detection of Calcium concentration, we either added Fluo-3 AM or transfected inverse-pericam into mouse embryonic stem cells, then treated some cells with histamine and others with cyproheptadine hydrochloride (Ca |
| 11:45, 15 March 2007 |  | 2,400 × 1,800 (779 KB) | Nzimm (talk | contribs) | MES cells + Fluo-3 AM + 0.1 uM cyproheptadine hydrochloride |
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