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(From: Front Microbiol. 2013; 4: 5. Published online 2013 January 25. doi: 10.3389/fmicb.2013.00005 Genetic safeguard strategies. Recombinant DNA (bright green) is introduced into the host chromosome (white wavy lines). Two pathways for engineered auxotro)
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From: Front Microbiol. 2013; 4: 5. Published online 2013 January 25. doi: 10.3389/fmicb.2013.00005

Genetic safeguard strategies. Recombinant DNA (bright green) is introduced into the host chromosome (white wavy lines). Two pathways for engineered auxotrophy (A,B) kill synthetic organisms (blue) once they lose access to a supplement (+) in a controlled environment. The supplement either (A) suppresses a toxic gene product (−) or (B) provides nutrition to compensate for a genetic deletion (red X). The induced lethality system (C) produces a toxic gene product (−) in response to an inducer (i) such as IPTG, sucrose, arabinose, or heat. Gene-flow prevention (D) is accomplished by placing a toxic gene into the recombinant DNA (dark blue/bright green circle) in an immune host. Transfer of the recombinant plasmid kills unintended host cells.

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14:26, 4 March 2013256×256 (9 KB)Thomas Wall (Talk | contribs) (From: Front Microbiol. 2013; 4: 5. Published online 2013 January 25. doi: 10.3389/fmicb.2013.00005 Genetic safeguard strategies. Recombinant DNA (bright green) is introduced into the host chromosome (white wavy lines). Two pathways for engineered auxotro)

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