IPTG
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Isopropyl-beta-D-thiogalactopyranoside
Induces transcription from promoters regulated by lacI repressor.
Molecular weight is 238.31 g/mol
- Dissolve 1g in 4196 μL deionized water to make 1M solution.
- Filter sterilize with syringe and 0.22μm filter
Generally a 1mM solution is an effective amount to induce the pLac promoter region. It should be noted that this may vary over cell strains. For example:
- lacIq: cell strains which overproduce lacI repressor (ie. E.coli cell strain type [[1]], aka. BBa_V1003)