IPTG

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Isopropyl-beta-D-thiogalactopyranoside

Induces transcription from promoters regulated by LacI repressor.

Molecular weight is 238.31 g/mol. The chemical formula is here.

  1. Dissolve 1g in 4196 μL deionized water to make 1M solution.
  2. Filter sterilize with syringe and 0.22μm filter

Generally a 1mM solution is an effective amount to induce the pLac promoter region. It should be noted that this may vary over cell strains. For example:

For including IPTG in LB agar plates, a typical amount people recommend is 0.1-0.5mM IPTG.

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