IPTG - Revision history

Christopher C Vanlang at 21:26, 23 December 2011

Christopher C Vanlang — Fri, 23 Dec 2011 21:26:39 GMT

←Older revision		Revision as of 21:26, 23 December 2011
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	Alternatively		Alternatively
-	#Dissolve 238 mg IPTG (sigma I-6758) in 10 ml DW	+	#Dissolve 238 mg IPTG (sigma I-6758) in 10 ml DW for (100mM stock)
	#store in 1 ml aliquotes at -20°C		#store in 1 ml aliquotes at -20°C

Christopher C Vanlang at 21:25, 23 December 2011

Christopher C Vanlang — Fri, 23 Dec 2011 21:25:42 GMT

←Older revision		Revision as of 21:25, 23 December 2011
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	#Dissolve 1g in 4196 μL deionized water to make 1M solution.		#Dissolve 1g in 4196 μL deionized water to make 1M solution.
	#Filter sterilize with syringe and 0.22μm filter		#Filter sterilize with syringe and 0.22μm filter
		+
		+	Alternatively
		+	#Dissolve 238 mg IPTG (sigma I-6758) in 10 ml DW
		+	#store in 1 ml aliquotes at -20°C

	Generally a 1mM solution is an effective amount to induce the pLac promoter region. It should be noted that this may vary over cell strains. For example:		Generally a 1mM solution is an effective amount to induce the pLac promoter region. It should be noted that this may vary over cell strains. For example:

Reshma P. Shetty at 20:09, 6 September 2008

Reshma P. Shetty — Sat, 06 Sep 2008 20:09:47 GMT

←Older revision		Revision as of 20:09, 6 September 2008
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	Generally a 1mM solution is an effective amount to induce the pLac promoter region. It should be noted that this may vary over cell strains. For example:		Generally a 1mM solution is an effective amount to induce the pLac promoter region. It should be noted that this may vary over cell strains. For example:
-	*lacIq: cell strains which overproduce LacI repressor (ie. [[E. coli genotypes\|E.coli cell strain]] type [[E. coli genotypes#D1210\|D1210]], aka. [[registry:Part:BBa_V1003\|BBa_V1003]])	+	*lacIq: cell strains which overproduce LacI repressor (ie. [[E. coli genotypes\|''E. coli'' cell strain]] type [[E. coli genotypes#D1210\|D1210]], aka. [[registry:Part:BBa_V1003\|BBa_V1003]])

	For including IPTG in LB agar plates, a typical amount people recommend is 0.1-0.5mM IPTG.		For including IPTG in LB agar plates, a typical amount people recommend is 0.1-0.5mM IPTG.

	[[Category:Material]] [[Category:Chemical]]		[[Category:Material]] [[Category:Chemical]]

Austin J. Che at 22:31, 3 April 2008

Austin J. Che — Thu, 03 Apr 2008 22:31:28 GMT

←Older revision		Revision as of 22:31, 3 April 2008
Line 10:		Line 10:
	Generally a 1mM solution is an effective amount to induce the pLac promoter region. It should be noted that this may vary over cell strains. For example:		Generally a 1mM solution is an effective amount to induce the pLac promoter region. It should be noted that this may vary over cell strains. For example:
	*lacIq: cell strains which overproduce LacI repressor (ie. [[E. coli genotypes\|E.coli cell strain]] type [[E. coli genotypes#D1210\|D1210]], aka. [[registry:Part:BBa_V1003\|BBa_V1003]])		*lacIq: cell strains which overproduce LacI repressor (ie. [[E. coli genotypes\|E.coli cell strain]] type [[E. coli genotypes#D1210\|D1210]], aka. [[registry:Part:BBa_V1003\|BBa_V1003]])
		+
		+	For including IPTG in LB agar plates, a typical amount people recommend is 0.1-0.5mM IPTG.

	[[Category:Material]] [[Category:Chemical]]		[[Category:Material]] [[Category:Chemical]]

Torsten Waldminghaus at 22:14, 5 March 2008

Torsten Waldminghaus — Wed, 05 Mar 2008 22:14:45 GMT

←Older revision		Revision as of 22:14, 5 March 2008
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	Isopropyl-beta-D-thiogalactopyranoside		Isopropyl-beta-D-thiogalactopyranoside

-	Induces transcription from promoters regulated by ~~lacI~~ repressor.	+	Induces transcription from promoters regulated by LacI repressor.

	Molecular weight is 238.31 g/mol. The chemical formula is [http://www.fermentas.com/catalog/reagents/img/iptg.gif here].		Molecular weight is 238.31 g/mol. The chemical formula is [http://www.fermentas.com/catalog/reagents/img/iptg.gif here].
Line 9:		Line 9:

	Generally a 1mM solution is an effective amount to induce the pLac promoter region. It should be noted that this may vary over cell strains. For example:		Generally a 1mM solution is an effective amount to induce the pLac promoter region. It should be noted that this may vary over cell strains. For example:
-	*lacIq: cell strains which overproduce ~~lacI~~ repressor (ie. [[E. coli genotypes\|E.coli cell strain]] type [[E. coli genotypes#D1210\|D1210]], aka. [[registry:Part:BBa_V1003\|BBa_V1003]])	+	*lacIq: cell strains which overproduce LacI repressor (ie. [[E. coli genotypes\|E.coli cell strain]] type [[E. coli genotypes#D1210\|D1210]], aka. [[registry:Part:BBa_V1003\|BBa_V1003]])

	[[Category:Material]] [[Category:Chemical]]		[[Category:Material]] [[Category:Chemical]]

Reshma P. Shetty at 17:23, 19 October 2007

Reshma P. Shetty — Fri, 19 Oct 2007 17:23:49 GMT

←Older revision		Revision as of 17:23, 19 October 2007
Line 10:		Line 10:
	Generally a 1mM solution is an effective amount to induce the pLac promoter region. It should be noted that this may vary over cell strains. For example:		Generally a 1mM solution is an effective amount to induce the pLac promoter region. It should be noted that this may vary over cell strains. For example:
	*lacIq: cell strains which overproduce lacI repressor (ie. [[E. coli genotypes\|E.coli cell strain]] type [[E. coli genotypes#D1210\|D1210]], aka. [[registry:Part:BBa_V1003\|BBa_V1003]])		*lacIq: cell strains which overproduce lacI repressor (ie. [[E. coli genotypes\|E.coli cell strain]] type [[E. coli genotypes#D1210\|D1210]], aka. [[registry:Part:BBa_V1003\|BBa_V1003]])
		+
		+	[[Category:Material]] [[Category:Chemical]]

Scmohr at 15:09, 14 August 2007

Scmohr — Tue, 14 Aug 2007 15:09:38 GMT

←Older revision		Revision as of 15:09, 14 August 2007
Line 3:		Line 3:
	Induces transcription from promoters regulated by lacI repressor.		Induces transcription from promoters regulated by lacI repressor.

-	Molecular weight is 238.31 g/mol	+	Molecular weight is 238.31 g/mol. The chemical formula is [http://www.fermentas.com/catalog/reagents/img/iptg.gif here].

	#Dissolve 1g in 4196 μL deionized water to make 1M solution.		#Dissolve 1g in 4196 μL deionized water to make 1M solution.

Austin J. Che at 20:35, 17 July 2006

Austin J. Che — Mon, 17 Jul 2006 20:35:15 GMT

←Older revision		Revision as of 20:35, 17 July 2006
Line 9:		Line 9:

	Generally a 1mM solution is an effective amount to induce the pLac promoter region. It should be noted that this may vary over cell strains. For example:		Generally a 1mM solution is an effective amount to induce the pLac promoter region. It should be noted that this may vary over cell strains. For example:
-	*lacIq: cell strains which overproduce lacI repressor (ie. [[E. coli genotypes\|E.coli cell strain]] type [[~~http://openwetware.org/wiki/~~E.~~_coli_genotypes~~#D1210\|D1210]], aka. [~~http~~:~~//parts.mit.edu/registry/index.php/~~Part:BBa_V1003 BBa_V1003])	+	*lacIq: cell strains which overproduce lacI repressor (ie. [[E. coli genotypes\|E.coli cell strain]] type [[E. coli genotypes#D1210\|D1210]], aka. [[registry:Part:BBa_V1003\|BBa_V1003]])

Melissali: added specific strain information

Melissali — Mon, 17 Jul 2006 19:48:05 GMT

added specific strain information

←Older revision		Revision as of 19:48, 17 July 2006
Line 8:		Line 8:
	#Filter sterilize with syringe and 0.22μm filter		#Filter sterilize with syringe and 0.22μm filter

-	Generally a 1mM solution is an effective amount to induce the pLac promoter region. ~~This~~ may vary over cell strains, ~~however~~.	+	Generally a 1mM solution is an effective amount to induce the pLac promoter region. It should be noted that this may vary over cell strains. For example:
		+	*lacIq: cell strains which overproduce lacI repressor (ie. [[E. coli genotypes\|E.coli cell strain]] type [[http://openwetware.org/wiki/E._coli_genotypes#D1210\|D1210]], aka. [http://parts.mit.edu/registry/index.php/Part:BBa_V1003 BBa_V1003])

Melissali at 00:47, 4 July 2006

Melissali — Tue, 04 Jul 2006 00:47:08 GMT

←Older revision		Revision as of 00:47, 4 July 2006
Line 7:		Line 7:
	#Dissolve 1g in 4196 μL deionized water to make 1M solution.		#Dissolve 1g in 4196 μL deionized water to make 1M solution.
	#Filter sterilize with syringe and 0.22μm filter		#Filter sterilize with syringe and 0.22μm filter
		+
		+	Generally a 1mM solution is an effective amount to induce the pLac promoter region. This may vary over cell strains, however.