IPTG: Difference between revisions
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#Dissolve 1g in 4196 μL deionized water to make 1M solution. | #Dissolve 1g in 4196 μL deionized water to make 1M solution. | ||
#Filter sterilize with syringe and 0.22μm filter | #Filter sterilize with syringe and 0.22μm filter | ||
Generally a 1mM solution is an effective amount to induce the pLac promoter region. This may vary over cell strains, however. | |||
Revision as of 17:47, 3 July 2006
Isopropyl-beta-D-thiogalactopyranoside
Induces transcription from promoters regulated by lacI repressor.
Molecular weight is 238.31 g/mol
- Dissolve 1g in 4196 μL deionized water to make 1M solution.
- Filter sterilize with syringe and 0.22μm filter
Generally a 1mM solution is an effective amount to induce the pLac promoter region. This may vary over cell strains, however.
