IPTG

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#Filter sterilize with syringe and 0.22μm filter
#Filter sterilize with syringe and 0.22μm filter
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Generally a 1mM solution is an effective amount to induce the pLac promoter region.  This may vary over cell strains, however.
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Generally a 1mM solution is an effective amount to induce the pLac promoter region.  It should be noted that this may vary over cell strains.  For example:
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*lacIq: cell strains which overproduce lacI repressor (ie. [[E. coli genotypes|E.coli cell strain]] type [[http://openwetware.org/wiki/E._coli_genotypes#D1210|D1210]], aka. [http://parts.mit.edu/registry/index.php/Part:BBa_V1003 BBa_V1003])

Revision as of 14:48, 17 July 2006

Isopropyl-beta-D-thiogalactopyranoside

Induces transcription from promoters regulated by lacI repressor.

Molecular weight is 238.31 g/mol

  1. Dissolve 1g in 4196 μL deionized water to make 1M solution.
  2. Filter sterilize with syringe and 0.22μm filter

Generally a 1mM solution is an effective amount to induce the pLac promoter region. It should be noted that this may vary over cell strains. For example:

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