IPTG

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#Dissolve 1g in 4196 μL deionized water to make 1M solution.
#Dissolve 1g in 4196 μL deionized water to make 1M solution.
#Filter sterilize with syringe and 0.22μm filter
#Filter sterilize with syringe and 0.22μm filter
 +
 +
Generally a 1mM solution is an effective amount to induce the pLac promoter region.  This may vary over cell strains, however.

Revision as of 20:47, 3 July 2006

Isopropyl-beta-D-thiogalactopyranoside

Induces transcription from promoters regulated by lacI repressor.

Molecular weight is 238.31 g/mol

  1. Dissolve 1g in 4196 μL deionized water to make 1M solution.
  2. Filter sterilize with syringe and 0.22μm filter

Generally a 1mM solution is an effective amount to induce the pLac promoter region. This may vary over cell strains, however.

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