IPTG

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Generally a 1mM solution is an effective amount to induce the pLac promoter region.  It should be noted that this may vary over cell strains.  For example:  
Generally a 1mM solution is an effective amount to induce the pLac promoter region.  It should be noted that this may vary over cell strains.  For example:  
*lacIq: cell strains which overproduce LacI repressor (ie. [[E. coli genotypes|E.coli cell strain]] type [[E. coli genotypes#D1210|D1210]], aka. [[registry:Part:BBa_V1003|BBa_V1003]])
*lacIq: cell strains which overproduce LacI repressor (ie. [[E. coli genotypes|E.coli cell strain]] type [[E. coli genotypes#D1210|D1210]], aka. [[registry:Part:BBa_V1003|BBa_V1003]])
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For including IPTG in LB agar plates, a typical amount people recommend is 0.1-0.5mM IPTG.
[[Category:Material]] [[Category:Chemical]]
[[Category:Material]] [[Category:Chemical]]

Revision as of 18:31, 3 April 2008

Isopropyl-beta-D-thiogalactopyranoside

Induces transcription from promoters regulated by LacI repressor.

Molecular weight is 238.31 g/mol. The chemical formula is here.

  1. Dissolve 1g in 4196 μL deionized water to make 1M solution.
  2. Filter sterilize with syringe and 0.22μm filter

Generally a 1mM solution is an effective amount to induce the pLac promoter region. It should be noted that this may vary over cell strains. For example:

For including IPTG in LB agar plates, a typical amount people recommend is 0.1-0.5mM IPTG.

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