IPTG

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
m
Line 3: Line 3:
Induces transcription from promoters regulated by lacI repressor.
Induces transcription from promoters regulated by lacI repressor.
-
Molecular weight is 238.31 g/mol
+
Molecular weight is 238.31 g/mol.  The chemical formula is [http://www.fermentas.com/catalog/reagents/img/iptg.gif here].
#Dissolve 1g in 4196 μL deionized water to make 1M solution.
#Dissolve 1g in 4196 μL deionized water to make 1M solution.

Revision as of 10:09, 14 August 2007

Isopropyl-beta-D-thiogalactopyranoside

Induces transcription from promoters regulated by lacI repressor.

Molecular weight is 238.31 g/mol. The chemical formula is here.

  1. Dissolve 1g in 4196 μL deionized water to make 1M solution.
  2. Filter sterilize with syringe and 0.22μm filter

Generally a 1mM solution is an effective amount to induce the pLac promoter region. It should be noted that this may vary over cell strains. For example:

Personal tools